Field of view programmable microscope device

Abstract

The invention discloses a field of view programmable microscope device. The field of view programmable microscope device comprises a PC, a control circuit, a light source, an optical filter, a uniform light bar, a prism lens set, a digital micromirror device, a lens array, a sample object table and a camera, wherein the light source is controlled by the PC for generating white light, special-wavelength light is generated from light through the optical filter, the point light source is expanded to be a plane light source to irradiate to the digital micromirror device through the light bar and the prism lens set, each mirror switch is controlled by the digital micromirror device for generating modulation light having different levels of brightness, shapes, regions, wavelengths, continuity time and scanning frequencies, to-be-detected samples are irradiated through the lens array, after images are acquired by the camera, the images are stored and displayed on the PC, and feedback adjustment is further realized. The device is advantaged in that accurate control on brightness, shape and wavelength of the light source can be realized, and the microscopic sample information is displayed or stored in the PC. Compared with a traditional microscope, a modulation mode and a scanning speed of space light can be freely controlled, the information can be displayed and stored, and high flexibility is realized.

Description

technical field [0001] The invention relates to the field of microscope illumination and the field of electronic technology, in particular to a field-of-view programmable microscope device for digital micromirror devices used in microscope illumination. The device can be used to acquire sample image data requiring different illumination brightness and illumination patterns. Background technique [0002] At present, with the development of research in biophysical chemistry and other fields and the advancement of science and technology, the application of microscopes is basically involved in all scientific research, and there are higher requirements for the observation mode of samples. When observing fluorescent samples, it is necessary to compare the samples with different illumination brightness, illumination pattern, illumination wavelength or illumination area; sometimes it is necessary to scan the sample at high speed to observe processes such as blood cell flow; in the ph...

AI Technical Summary

Problems solved by technology

[0003] The spatial light modulation of traditional microscopes is realized through pinhole arrays and lens arrays. Once the design and manufacture are completed, it can no longer be changed. When adjusting parameters, you can only choose to replace the device, which is very uncomfortable in the actual use process. convenient

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