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A kind of preparation method of egg yolk antibody with high titer

A yolk antibody, high-titer technology, applied in the direction of anti-bacterial immunoglobulin, egg-derived immunoglobulin, etc., can solve the problems of high lethality, unsatisfactory effect, and acute onset, and achieve rapid curative effect, low price, and easy saved effect

Inactive Publication Date: 2017-06-09
TIANJIN ZHENBANG AQUACULTURE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for individuals who have not been vaccinated, when they are infected with the above-mentioned diseases, clinically, symptomatic treatment and antibiotics are mainly used to prevent complications.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A method for preparing a high-titer egg yolk antibody, comprising the following steps:

[0016] 1) Separation of high-free egg yolks: sterilize high-free eggs that have been immunized with Pasteurella inactivated vaccines, and then send them to egg white and yolk separators for egg yolk separation;

[0017] 2) Preparation of purified egg yolk antibody: add acetic acid buffer to the egg yolk separated in step 1) for dilution, wherein the volume ratio of egg yolk to acetic acid buffer is 1:5, adjust the pH to 4.5 with acid, and precipitate at 4°C; The ratio of adding 16 μL caprylic acid per milliliter of diluted egg yolk liquid was added dropwise under stirring at room temperature, and the addition was completed within 30 minutes, and then stood at low temperature for 2 hours, and then centrifuged at a speed of 10000r / min for 30 minutes, discarding the precipitate; after filtering the supernatant, Add 1 / 10 volume of 0.01mol / L PBS, adjust the pH to 7.2 with alkali; mix 40%...

Embodiment 2

[0022] A method for preparing a high-titer egg yolk antibody, comprising the following steps:

[0023] 1) Separation of high-free egg yolks: sterilize high-free eggs that have been immunized with Pasteurella inactivated vaccines, and then send them to egg white and yolk separators for egg yolk separation;

[0024] 2) Preparation of purified egg yolk antibody: add acetic acid buffer to the egg yolk separated in step 1) for dilution, wherein the volume ratio of egg yolk to acetic acid buffer is 1:5, adjust the pH to 4.5 with acid, and precipitate at 4°C; The ratio of adding 16 μL caprylic acid per milliliter of diluted egg yolk liquid was added dropwise under stirring at room temperature, and the addition was completed within 30 minutes, and then stood at low temperature for 2 hours, and then centrifuged at a speed of 10000r / min for 30 minutes, discarding the precipitate; after filtering the supernatant, Add 1 / 10 volume of 0.01mol / L PBS, adjust the pH to 7.2 with alkali; mix 40%...

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PUM

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Abstract

The invention provides a method for preparing a high-titer egg yolk antibody. The method of the invention is used to obtain a higher titer egg yolk antibody by adjusting the process characteristics, which has specificity, good therapeutic effect and rapid curative effect on piglet Pasteurella disease. The preparation can effectively prevent and treat pasteurellosis in piglets; its preparation method adopts octanoic acid precipitation method to extract refined egg yolk antibodies against Pasteurella piglets, which is safe, low in price, suitable for large-scale production, and the prepared egg yolk antibodies are pure High, high potency, stable properties, easy to store.

Description

technical field [0001] The invention relates to the technical field of antibody preparation, in particular to a method for preparing a high-titer yolk antibody. Background technique [0002] Pasteurella is a sporeless, non-motile, facultative anaerobic, Gram-negative small bacillus that often stains heavily at both ends of the bacteria. It was named after Pasteurella multocida in this genus was first isolated from sick chickens by Pasteur in 1880. Yersinia pestis, Pseudotuberculosis bacillus, Enterocolitis bacillus and Tularensis originally belonged to this genus, but now the first three have been reclassified to Yersinia, and the last one has been reclassified to Francisella. The identified species of this genus are Pasteurella multocida, Pasteurella pneumophila, Pasteurella hemolyticus, Pasteurella urinae and Pasteurella anatipestifer. [0003] Pasteurella suis is currently mainly immunized with vaccines to prevent the occurrence of the disease. By immunizing pregnant so...

Claims

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Application Information

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IPC IPC(8): C07K16/12C07K16/02
Inventor 季延滨李涛侯继明
Owner TIANJIN ZHENBANG AQUACULTURE
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