Detection kit for activity and total amount of lipoprotein-associated phospholipase Lp-PLA2 and preparation method thereof

Abstract

The invention discloses a detection kit and a detection method for the activity and the total amount of lipoprotein-associated phospholipase Lp-PLA2. The detection kit is prepared from a solid-phase carrier, a zymolytic substrate, a buffer solution, an enzyme labeled antibody reagent, a cleaning solution, a chromogenic substrate, a stop solution, an enzymatic activity calibration substance and an enzymatic calibration-free substance, wherein the enzyme-linked immunosorbent assay plate is coated with an anti-Lp-PLA2 specific N-terminal non-inhibiting antibody; the zymolytic substrate is a platelet activating factor, a platelet activating factor analogue or phosphatidylcholine modified by the platelet activating factor analogue in an oxidative manner; the enzyme labeled antibody reagent is horseradish peroxidase or alkaline phosphatase or a biotin labeled anti-Lp-PLA2 inhibiting antibody; the enzymatic activity calibration substance comprises multiple concentrations of paranitrophenol standard substances; the enzymatic calibration-free substance comprises multiple reference calibration substances containing Lp-PLA2 antigens. By using the detection kit, the inference of other matter in a sample can be avoided; the in-vivo condition of the Lp-PLA2 can be more accurately reflected; the more accurate judgment basis is provided for clinic.

Description

technical field [0001] The invention relates to the field of in vitro diagnosis, in particular to a lipoprotein-related phospholipase Lp-PLA 2 Activity and total amount detection kit and preparation method thereof. Background technique [0002] According to the statistics of the World Health Organization, more than 3 million people die of cardiovascular and cerebrovascular diseases in China every year, and one person dies of cardiovascular and cerebrovascular diseases every 10 seconds. Far more than other diseases such as cancer. With the continuous aging of the population in our country, the number of people suffering from cardiovascular and cerebrovascular diseases is increasing year by year, and tends to be younger; the number of patients in my country has reached more than 60 million, and stroke patients reach 2 million every year, with an incidence rate as high as 120 / 10 It is a common disease that often occurs in elderly men over 55 years old, women over 65 years old ...

AI Technical Summary

Problems solved by technology

The disadvantage of this scheme is that some blood components directly affect the detection of LP-PLA 2 The result of enzyme activity, such as heme, bilirubin, metabolites, drugs, etc.; there is also LP-PLA in blood or cell fluid 2 Similar to PLA 2 The presence of phospholipase can also hydrolyze LP-PLA 2 Enzyme substrate, lower detectable blood LP-PLA 2 Specificity of Enzyme Activity

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