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Cigarette main stream smoke nicotine chiral analysis combined-phase chromatography-tandem mass spectrometry

A cigarette mainstream smoke and convergence chromatography technology, which is applied in the field of determination of chemical components of cigarette mainstream smoke, can solve the problems of complex processing steps and inability to meet the detection of a large number of samples, and achieve high sensitivity, good specificity, and pollution reduction effects

Active Publication Date: 2017-06-30
CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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Problems solved by technology

[0003] At present, the chiral analysis of nicotine is mainly studied by gas chromatography. For example, the patent application number 201610663534.1 "A Method for Chiral Analysis of Nicotine in Cigarette Tobacco" has complicated pretreatment steps and a long detection time. Up to 140min, which cannot meet the detection of a large number of samples. At present, there is no method for chiral analysis of nicotine in mainstream cigarette smoke

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  • Cigarette main stream smoke nicotine chiral analysis combined-phase chromatography-tandem mass spectrometry
  • Cigarette main stream smoke nicotine chiral analysis combined-phase chromatography-tandem mass spectrometry
  • Cigarette main stream smoke nicotine chiral analysis combined-phase chromatography-tandem mass spectrometry

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Embodiment Construction

[0015] The present invention is further described by the following specific examples, but does not limit the present invention.

[0016] 1. Instruments and Reagents

[0017] ACQUITY UPC2 Ultra-High Performance Convergence Chromatograph (Waters, USA), 6410B triple quadrupole mass spectrometer (Agilent, USA), AE163 electronic balance (sensitivity: 0.0001 g, Mettler, Switzerland), high-speed grinder (Wuhan Yincai) Technology Co., Ltd.), American Coleparmer Votex-Genie vortex shaker, Germany SIGMA 3-30K-high-speed desktop refrigerated centrifuge. Methyl tert-butyl ether (chromatographic grade), sodium hydroxide (analytical grade), formic acid (chromatographic grade), methanol (chromatographic grade), S-(-)-nicotine standard (CAS: 54-11-5) , R-(+)-nicotine standard (CAS: 25162-00-9).

[0018] 2. Preparation of standard solutions

[0019] Weigh about 100.0 mg of S-(-)-nicotine standard, dilute it with methanol solution to a 50 mL brown volumetric flask, and prepare a S-(-)-nicoti...

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Abstract

The invention discloses a cigarette main stream smoke nicotine chiral analysis combined-phase chromatography-tandem mass spectrometry. The cigarette main stream smoke nicotine chiral analysis combined-phase chromatography-tandem mass spectrometry is characterized by comprising collecting nicotine in mainstream smoke of a smoked cigarette sample through a Cambridge filter, soaking the Cambridge filter in sodium hydroxide solution, performing extraction through methyl tertiary butyl ether and methanol solution, performing matrix solid-phase disperse extraction and then purification on liquid extract, performing combined-phase chromatography-tandem mass spectrometry through two chiral columns, quantitatively detecting the proportion of S-(-)-nicotine to R-(+)-nicotine in the cigarette main stream smoke. The cigarette main stream smoke nicotine chiral analysis combined-phase chromatography-tandem mass spectrometry is simple, efficient, high in sensitivity and specificity, capable of eliminating false positive and low in analyzing time consumption, and can reduce the contamination of sample liquor to chromatographic columns and meet the chiral analysis on nicotine in a large batch of cigarette main stream samples.

Description

technical field [0001] The invention relates to a method for determining the chemical components of mainstream cigarette smoke, in particular to a chiral analysis of nicotine in mainstream cigarette smoke, a convergent phase chromatography tandem mass spectrometry method. Background technique [0002] Nicotine, also called nicotine, is an alkaloid found in the nightshade family and an important component of tobacco. The nicotine molecule contains a chiral center, the 2-position carbon atom on the tetrahydropyrrole ring, so nicotine has two enantiomers: S-(-)-nicotine and R-(+)-nicotine. The two enantiomers of nicotine have completely different contents, metabolic mechanisms and physiological properties. Therefore, chiral analysis of nicotine in mainstream cigarette smoke is required. [0003] At present, the chiral analysis of nicotine is mainly carried out by gas chromatography. For example, the patent application No. 201610663534.1 "A Method for Chiral Analysis of Nicoti...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14
CPCG01N30/02G01N30/06G01N30/14G01N2030/062
Inventor 庞永强费婷张洪非陈欢吴达戚大伟姜兴益朱风鹏罗彦波李翔宇胡少东付亚宁韩书磊
Owner CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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