Cigarette main stream smoke nicotine chiral analysis combined-phase chromatography-tandem mass spectrometry

Abstract

The invention discloses a cigarette main stream smoke nicotine chiral analysis combined-phase chromatography-tandem mass spectrometry. The cigarette main stream smoke nicotine chiral analysis combined-phase chromatography-tandem mass spectrometry is characterized by comprising collecting nicotine in mainstream smoke of a smoked cigarette sample through a Cambridge filter, soaking the Cambridge filter in sodium hydroxide solution, performing extraction through methyl tertiary butyl ether and methanol solution, performing matrix solid-phase disperse extraction and then purification on liquid extract, performing combined-phase chromatography-tandem mass spectrometry through two chiral columns, quantitatively detecting the proportion of S-(-)-nicotine to R-(+)-nicotine in the cigarette main stream smoke. The cigarette main stream smoke nicotine chiral analysis combined-phase chromatography-tandem mass spectrometry is simple, efficient, high in sensitivity and specificity, capable of eliminating false positive and low in analyzing time consumption, and can reduce the contamination of sample liquor to chromatographic columns and meet the chiral analysis on nicotine in a large batch of cigarette main stream samples.

Description

technical field [0001] The invention relates to a method for determining the chemical components of mainstream cigarette smoke, in particular to a chiral analysis of nicotine in mainstream cigarette smoke, a convergent phase chromatography tandem mass spectrometry method. Background technique [0002] Nicotine, also called nicotine, is an alkaloid found in the nightshade family and an important component of tobacco. The nicotine molecule contains a chiral center, the 2-position carbon atom on the tetrahydropyrrole ring, so nicotine has two enantiomers: S-(-)-nicotine and R-(+)-nicotine. The two enantiomers of nicotine have completely different contents, metabolic mechanisms and physiological properties. Therefore, chiral analysis of nicotine in mainstream cigarette smoke is required. [0003] At present, the chiral analysis of nicotine is mainly carried out by gas chromatography. For example, the patent application No. 201610663534.1 "A Method for Chiral Analysis of Nicoti...

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Problems solved by technology

[0003] At present, the chiral analysis of nicotine is mainly studied by gas chromatography. For example, the patent application number 201610663534.1 "A Method for Chiral Analysis of Nicotine in Cigarette Tobacco" has complicated pretreatment steps and a long detection time. Up to 140min, which cannot meet the detection of a large number of samples. At present, there is no method for chiral analysis of nicotine in mainstream cigarette smoke

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