A kind of breeding method of high-zinc and pest-resistant green rice

Abstract

The invention discloses a method for breeding high-zinc-content, disease-resistant, insect-resistant and environment-friendly rice. The method comprises the steps: selecting disease-resistant rice as a parent plant I, selecting expelling type insect-resistant rice as a parent plant II, and carrying out cross breeding, so as to obtain single-cross F1 seeds; selecting non-glutinous rice with the zinc content of 30mg / kg or more as a parent plant III, planting a single-cross F1 generation, and carrying out cross breeding on the single-cross F1 generation and the parent plant III, so as to obtain three-way-cross F1 seeds; planting a three-way-cross F1 generation, and constructing doubled haploid populations by adopting an anther culture technique; planting a DH1 generation according to plant lines, extracting DNA from the DH1 generation, carrying out molecular marker identification to select disease-resistant genes, and selecting plant lines, of which polished rice has the zinc content not lower than 30mg / kg, from selected plant lines; planting a DH2 generation according to plant lines, and selecting plant lines which have expelling type insect resistance to rice planthoppers and striped rice borers; planting a DH3 generation according to strains, and selecting japonica-cline type strains; and planting a DH4 generation according to strains, and selecting disease-resistant, insect-resistant and japonica-cline type stereotyped strains with the zinc content of 30mg / kg or more, thereby obtaining the high-zinc-content, disease-resistant, insect-resistant and environment-friendly rice. The bred rice variety integrates 3 kinds of excellent features, i.e., high zinc content, disease resistance and insect resistance.

Description

technical field [0001] The invention relates to the technical field of rice breeding, in particular to a method for breeding green rice with high zinc content and resistance to diseases and insect pests. Background technique [0002] Zinc is an essential micronutrient element for the human body and has important physiological functions. Zinc deficiency in the human body will cause a series of metabolic disorders and directly endanger human health. For example, zinc deficiency in children will cause growth retardation and immune function reduction in mild cases, and mental retardation and even death in severe cases. Zinc is mainly found in seafood and animal offal, and other foods contain very little zinc. In the daily diet, whether it is rice, noodles, bread and other staple foods, or children's favorite milk, egg foods and other snacks, the zinc content is very low, and there are very few zinc-rich vegetables and fruits. WHO and FAO recommend that the daily zinc intake sh...

AI Technical Summary

Problems solved by technology

Frequent outbreaks of original diseases and insect pests such as rice blast, rice planthopper and rice stem borer have caused huge economic losses. Serious damage to rice production, hygiene and safety quality due to untimely prevention has become very common