Application of miR-31-5p and miR-99a-5p in distinguishing active tuberculosis and latent tuberculosis infection

Abstract

The invention discloses application of miR-31-5p and miR-99a-5p in distinguishing active tuberculosis and latent tuberculosis infection. Substances for detecting the expression quantity of miR-31-5p comprise a reagent and an instrument which are used for detecting the expression quantity of the miR-31-5p by using quantitative PCR (polymerase chain reaction) and a reagent and an instrument which are used for detecting the expression quantity of the miR-31-5p by using an RNA-Seq; and substances for detecting the expression quantity of miR-99a-5p comprise a reagent and an instrument which are used for detecting the expression quantity of the miR-99a-5p by using the quantitative PCR and a reagent and an instrument which are used for detecting the expression quantity of the miR-99a-5 by using the RNA-Seq. The AUC of the miR-31-5p and the miR-99a-5p which are used for distinguishing an active tuberculosis patient and a latent tuberculosis infection patient is greater than 0.8, and the specificity is high; and the miR-31-5p and the miR-99a-5p can be used for distinguishing the active tuberculosis and the latent tuberculosis infection.

Description

Technical field [0001] The invention relates to the application of miR-31-5p and miR-99a-5p in distinguishing active tuberculosis and tuberculosis latent infection in the field of biotechnology. Background technique [0002] Tuberculosis (Tuberculosis, TB) is a chronic infectious disease caused by Mycobacterium tuberculosis (MTB) infection, which seriously threatens human health. The fifth national tuberculosis epidemiological survey showed that there are more than 5 million adult patients with active tuberculosis in China. After adults are infected with Mycobacterium tuberculosis, some of them will be living with the bacteria and will not develop into tuberculosis, which is called latent tuberculosis infection (LTBI); about 5-10% of people with latent infection will continue to develop active Tuberculosis (Active tuberculosis, ATB). Active pulmonary tuberculosis means that the tuberculosis lesions are in the active period, and there are often patchy shadows or tuberculosis cav...

AI Technical Summary

Problems solved by technology

Although the sputum smear staining method can produce results on the same day, it cannot distinguish tuberculosis bacilli from non-tuberculous mycobacteria, nor can it distinguish whether MTB is a living or dead bacteria

Although the sensitivity of Mycobacterium tuberculosis culture method is higher, it takes a long time, and it takes 1 month to get the result even for rapid culture.

In addition, for a large number of smear-negative and bacterium-negative active pulmonary tuberculosis patients, the application of gold standard detection methods is limited, which exacerbates the difficulty of diagnosis and brings resistance to timely treatment

Although some advanced nucleic acid amplification-based molecular biology detection techniques (such as XpertMTB / RIF assay) have emerged in recent years, they cannot be fully promoted due to the limitations of equipment and diagnostic costs.

Therefore, none of the current tuberculosis diagnostic methods or auxiliary diagnostic methods can achieve rapid and effective differential diagnosis of latent infection of Mycobacterium tuberculosis and active tuberculosis, which makes clinical problems such as serious delays in treatment and overtreatment

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