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Deep cryogenic freezing method for tumor tissue blocks

A technology of tumor tissue and freezing method, which is applied in the preservation, application, animal husbandry and other directions of human or animal body, can solve the problems of low tumor formation rate and reduced tumor cell activity, and achieves maintenance of cell viability, good activity, and easy removal. Effect

Active Publication Date: 2017-08-15
天晴干细胞股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention is to solve the problem that the existing tissue cryopreservation method leads to a significant decrease in tumor cell activity and low tumor formation rate after resuscitation, and provides a deep-low temperature freezing method for tumor tissue blocks

Method used

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  • Deep cryogenic freezing method for tumor tissue blocks
  • Deep cryogenic freezing method for tumor tissue blocks
  • Deep cryogenic freezing method for tumor tissue blocks

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specific Embodiment approach 1

[0025] Specific embodiment 1: The cryogenic freezing method of tumor tissue blocks in this embodiment includes the following steps:

[0026] 1. Preparation of cryopreservation solution:

[0027] Use DMEM to prepare freeze-dried human serum albumin powder into a human serum albumin solution with a mass concentration of 10% to 40%, and use DMEM to prepare hydroxyethyl starch into a hydroxyethyl starch solution with a mass concentration of 15% to 25%. , use DMEM to prepare ethylene glycol into ethylene glycol solutions with a mass concentration of 20% to 30%, and filter the three solutions under sterile conditions with 0.22 μm filters for use;

[0028] Then human serum albumin solution, hydroxyethyl starch solution, ethylene glycol solution and dimethyl sulfoxide are (25-30): (10-15): (8-12): (1-1.5 ) were mixed in proportion to obtain a frozen storage solution, which was pre-cooled at 4°C for later use;

[0029] 2. Processing of organization blocks:

[0030] After rinsing the...

specific Embodiment approach 2

[0034] Specific embodiment two: the difference between this embodiment and specific embodiment one is: in the step one, a human serum albumin solution with a mass concentration of 20% to 30% is prepared. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0035] Specific embodiment three: what this embodiment is different from specific embodiment one or two is: make mass concentration in step one and be 18% hydroxyethyl starch solution. Others are the same as in the first or second embodiment.

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Abstract

The invention provides a deep cryogenic freezing method for tumor tissue blocks, and relates to tumor tissue freezing methods. The deep cryogenic freezing method is used for solving the problems of the existing tissue cryopreservation methods that the activity of resuscitated tumor cells is remarkably lowered and the tumor forming rate is low. The method comprises the following steps: (1) preparing a cryopreservation solution; (2) treating and freezing the tissue blocks; (3) resuscitating cryopreserved tumor tissue blocks. According to the method, tumor tissue cryopreservation is carried out by using a permeable and impermeable cryopreservation protectant combined method, meanwhile, a preparing proportion of reagents is optimized, the proportion of DMSO in the cryopreservation solution is lowered to 2%, the cytotoxicity of tissue is lowered, a very good protective action on the cryopreserved tissue is achieved, thus, the cryopreserved tissue has relatively high mouse tumor forming performance after resuscitation, and the tumor forming rate reaches 90% or more. The method is applied to the freezing of the tumor tissue.

Description

technical field [0001] The invention relates to a tumor tissue freezing method. Background technique [0002] Tumor surface markers are specific substances produced during the growth of tumor cells, or cellular components overexpressed due to cytopathic changes. Pathological diagnosis and typing can provide a rationalization plan for tumor treatment. Therefore, when changing the treatment plan, you should Refer to the existing pathological results or re-perform pathological detection and analysis in other aspects. There are many tumor therapeutic vaccines currently under study, and clinical research is active. As of January 2017, 1,862 clinical studies have been registered on the ClinicalTrials.gov website in the United States, including 71 in China. Therapeutic vaccines that can mediate strong anti-tumor effects in vivo mainly include: 1) Recombinant tumor-associated antigens (TAA), which have combinations of short synthetic peptides or full-length proteins, the former nee...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0284
Inventor 刘春香张怡刘艳青
Owner 天晴干细胞股份有限公司
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