Universal test primer for viruses in tobamovirus and method for universal test primer
A technology for general detection of tobacco mosaic virus, applied in the fields of molecular biology and virology, can solve the problems of heavy workload, no virus, high cost, etc., and achieve the effect of low cost, high sensitivity and high detection efficiency
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[0021] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the embodiments of the present invention and the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.
[0022] Such as figure 1 Shown, a kind of tobacco mosaic virus genus virus universal detection primer sequence is:
[0023] Forward primer TobamodF: 5'-TKGAYGGNGTBCCNGGNTGYGG-3',
[0024] Reverse primer TobamodR: 5'-ACNGAVTBNABCTGTAATTGCTAT-3';
[0025] The primers amplify the product of 871bp to TMGMV, amplify the product of 877bp to PMMoV, amplify the product of 880bp to TMV, ToMV, ToMMV, amplify the product of 889bp to ORSV, and amplify the pro...
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