Universal test primer for viruses in tobamovirus and method for universal test primer

A technology for general detection of tobacco mosaic virus, applied in the fields of molecular biology and virology, can solve the problems of heavy workload, no virus, high cost, etc., and achieve the effect of low cost, high sensitivity and high detection efficiency

Inactive Publication Date: 2017-08-18
YUNNAN AGRICULTURAL UNIVERSITY
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Problems solved by technology

Tobacco mosaic virus has many types and strong infectivity, especially in Solanaceae crops, but so far there is no general detection primer for this genus virus, and a single specific detection primer is used to detect the infection of suspected viruses. Large volume, high cost and cumbersome

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  • Universal test primer for viruses in tobamovirus and method for universal test primer
  • Universal test primer for viruses in tobamovirus and method for universal test primer

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Embodiment Construction

[0021] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the embodiments of the present invention and the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0022] Such as figure 1 Shown, a kind of tobacco mosaic virus genus virus universal detection primer sequence is:

[0023] Forward primer TobamodF: 5'-TKGAYGGNGTBCCNGGNTGYGG-3',

[0024] Reverse primer TobamodR: 5'-ACNGAVTBNABCTGTAATTGCTAT-3';

[0025] The primers amplify the product of 871bp to TMGMV, amplify the product of 877bp to PMMoV, amplify the product of 880bp to TMV, ToMV, ToMMV, amplify the product of 889bp to ORSV, and amplify the pro...

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Abstract

The invention belongs to the technical field of molecular biology and virology and particularly relates to a universal test primer for viruses in a tobamovirus and a method for the universal test primer. Primer pair sequences of the universal test primer for the viruses in the tobamovirus are a forward primer TobamodF:5'-TKGAYGGNGTBCCNGGNTGYGG-3', and a reverse primer TobamodR:5'-ACNGAVTBNABCTGTAATTGCTAT-3. The test primer is designed on the basis of 32 conserved region sequences of a virus genome in the tobamovirus, amplification effect verification is carried out on the pair of primers by using seven viruses in the tobamovirus, and the amplification effects all are relatively good, thereby building universal test primer and method capable of testing at least seven viruses in the tobamovirus. The universal test primer has the characteristics of being high in testing efficiency, high in sensitivity and low in cost.

Description

technical field [0001] The invention belongs to the technical fields of molecular biology and virology, and in particular relates to a universal detection primer for tobacco mosaic virus and a method thereof. Background technique [0002] Tobamovirus is a genus with the largest number of virus species in Virgaviridae. According to the report of the Ninth Congress of the International Committee on Taxonomy of Viruses (ICTV) released in 2012, Tobamovirus has 25 confirmed species and 6 tentative species, whose representative species is TMV. According to the latest online report of ICTV, Tobamovirus has 35 kinds of viruses (http: / / www.ictvonline.org / virusTaxonomy.asp). Tobacco mosaic virus is distributed all over the world. It can infect important crops such as Solanaceae, Brassicaceae, and Cucurbitaceae and cause serious damage. It is one of the most economically important virus genera. [0003] So far, there are general detection primers for virus genera such as Potyvirus, B...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68C12R1/94
CPCC12Q1/701
Inventor 李凡李月月谭冠林肖龙马龑熊锋兰平秀陈小姣何英云谭松涛
Owner YUNNAN AGRICULTURAL UNIVERSITY
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