Primer pair, kit, method for identifying true and false of cordycepssinensis and application thereof

A technology of Cordyceps sinensis and primer pair, applied to a PCR method for identifying the authenticity of Cordyceps sinensis and the field of identifying the authenticity of Cordyceps sinensis, can solve the problem of poor stability of amplification effect, can not really reflect the authenticity of Cordyceps sinensis, and the amplification band of specific primers is weak. To achieve the effect of wide identification range, stable and reliable identification method and strong specificity

Pending Publication Date: 2019-05-31
DONGGUAN HEC CORDYCEPS R&D CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that it is not enough to judge the authenticity of Cordyceps sinensis only by identifying fungi or insects, and it cannot truly reflect the authenticity of the complex of Cordyceps sinensis
Although the name of the invention is "a double PCR detection method for authenticity identification of Cordyceps sinensis" and the application number is CN201710284515.2, the patent discloses a double primer using insects and bacteria to identify the authenticity of Cordyceps sinensis, but its insects The amplified bands of the specific primers are weak, and the stability of the amplification effect is poor

Method used

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  • Primer pair, kit, method for identifying true and false of cordycepssinensis and application thereof
  • Primer pair, kit, method for identifying true and false of cordycepssinensis and application thereof
  • Primer pair, kit, method for identifying true and false of cordycepssinensis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0091] Example 1 Primer pairs CC-2F / CC-2R to identify Cordyceps sinensis and counterfeit products

[0092] 1.1 Solution preparation

[0093] (1) Preparation of test sample DNA extract

[0094] Take about 30mg of the test product powder into a 2mL centrifuge tube, extract DNA strictly according to the instructions of the Tiangen Plant Genomic DNA Extraction Kit, pack the extract into 5μL / tube, and store it at -20°C for later use. The test products were 3 copies each of Cordyceps sinensis CSC1, CSC2, CSC3, CSC4 and CSC5, and counterfeit products of Cordyceps sinensis LS, DS, YXB, XJ, BC, DC, CH, YCC, CCH, BL, JSB, ZH-1, ZH- 2. One copy of each BFE.

[0095] (2) Preparation of Positive Control Substance Solution

[0096] Take about 30 mg of Cordyceps sinensis control medicinal material into a 2 mL centrifuge tube, extract DNA strictly according to the instructions of Tiangen Plant Genomic DNA Extraction Kit, divide the extract into 5 μL / tube, and store it at -20°C for later us...

Embodiment 2 2

[0105] Example 2 Double Identification Primer pairs CC-2F / CC-2R and DCF4 / DCR4 to identify Cordyceps sinensis and counterfeit products

[0106] 2.1 Solution preparation

[0107] (1) Preparation of test sample DNA extract

[0108] Take about 30mg of the test product powder into a 2mL centrifuge tube, extract DNA strictly according to the instructions of the Tiangen Plant Genomic DNA Extraction Kit, pack the extract into 5μL / tube, and store it at -20°C for later use. The test products are Cordyceps CSC1, CSC2, CSC3, CSC5, wild Cordyceps SC-1, SC-2, SC-3, SC-4, XZ-1, XZ-2, XZ-3, XZ-4, QH-1 , QH-2, QH-3, QH-4, counterfeit Cordyceps sinensis LS, DS, YXB, XJ, DC, CH, YCC, CCH, JSB, BC.

[0109] (2) Preparation of Positive Control Substance Solution

[0110] Take about 30 mg of Cordyceps sinensis control medicinal material into a 2 mL centrifuge tube, extract DNA strictly according to the instructions of Tiangen Plant Genomic DNA Extraction Kit, divide the extract into 5 μL / tube, a...

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Abstract

The invention belongs to the field of identification of Chinese medicinal materials, relating to a primer pair, kit and method and application thereof for identifying true and false of cordycepssinensis.In particular, the invention relates to the primer pair for identifying true and false of cordycepssinensis, which comprises CC-2F:5'-ATTAAGTCGTGGAAATG-3', CC-2R:5'-GATCAGGAATAGTGGGA-3', the kit thereof and the method for identifying the authenticity of the cordycepssinensis by PCR amplification by using the primer pair.The method has strong specificity and wider detection range.The invention also relates to the method for identifying the true and false of the cordycepssinensis by using the double primer pairs CC-2F/ CC-2R and DCF4/ DCR4 through PCR amplification, the amplification effect of the method is more obvious, the identification method is stable and reliable, and the identification range is wide.

Description

technical field [0001] The invention belongs to the field of identification of traditional Chinese medicinal materials, and in particular relates to a pair of COI-specific primers for identifying the authenticity of Cordyceps sinensis, a kit containing them, a PCR method for identifying the authenticity of Cordyceps sinensis and its application in identifying the authenticity of Cordyceps sinensis. Background technique [0002] Cordyceps sinensis (Ophiocordyceps sinensis sym.Cordyceps) is a complex of the sub-seats (fruiting bodies) and larval corpses of the ergotaceae fungus Cordyceps sinensis parasitizing on the larvae of the bat moth larvae of the Lepidoptera bat moth family. It is mainly distributed in the Qinghai-Tibet Plateau above 3000 Areas above the snow line. As early as in the "Compendium of Materia Medica", it is recorded: "Cordyceps sinensis, Ganping, protects the lungs and kidneys, stops bleeding and resolves phlegm, and relieves cough." The resource is scarce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/686C12N15/11
Inventor 钱正明李春红黄琦谢美霞李文佳
Owner DONGGUAN HEC CORDYCEPS R&D CO LTD
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