A method for identifying the drought resistance of maize varieties based on real-time quantitative PCR

Abstract

The invention discloses a method for identifying the drought resistance of corn varieties based on real-time quantitative PCR, which is to subject the corn varieties to drought stress during the seedling growth process, set a control group at the same time, extract the total RNA of the corn plants, and analyze the DNAJ gene expression was detected to obtain the expression drought resistance index DI E ; Use DI E Expression drought resistance index was used to evaluate the drought resistance performance of maize. The invention can realize rapid and accurate identification of the drought resistance of maize, and provide technical support for the screening of the drought resistance of maize.

Description

technical field [0001] The invention belongs to the field of drought resistance identification of corn varieties, in particular to a method for identifying drought resistance of corn varieties based on real-time quantitative PCR. Background technique [0002] Maize is the most productive food crop in the world, and its productivity is seriously threatened by drought. The drought resistance performance of maize is restricted by its own genetic effects and environmental factors. Due to the different growth and development stages of crops, the influence of biological factors and abiotic factors, any single drought resistance research has certain limitations so far, and it is difficult to Direct and accurate evaluation of drought resistance of maize. There are many methods to identify the drought resistance of maize, mainly including field direct identification method, artificial simulated environment method, physiological index method, molecular biological identification metho...

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Problems solved by technology

Among them, the field direct identification method is to identify the growth form of crops in different growth and development periods. However, these methods have a long time and are restricted by environmental factors, especially the inter-annual precipitation. and other shortcomings; the artificial simulated environment method refers to artificially creating the drought stress environment required for the experiment by adjusting the moisture content of the soil and air in the arid shed, growth box or artificial climate chamber, and by studying the growth and development, physiological process or The method of evaluating the drought resistance of water corn by the change of yield results, this method requires certain equipment, and the energy consumption is relatively large. There are some differences in the results of direct identification; the identification of plant drought resistance by physiological and biochemical indicators is mainly based on the indicators related to leaf water, plasma membrane permeability and enzyme activity, etc. However, these physical and chemical indicators will vary depending on the growth environment and growth period. Changes are also likely to cause errors due to the reagents used and human operations; the molecular biology identification method refers to the use of relevant molecular markers to construct a saturated molecular genetic map based on modern molecular biology, and to conduct drought-resistant genes in maize. Positioning, using molecular markers to select drought-resistant varieties of maize. Drought resistance is a quantitative trait controlled by multiple genes. Many drought-resistant QTLs have been discovered in recent years, but the polymorphism frequency of the markers is very low. A single QTL has little effect on phenotypic differences. The use of molecular marker-assisted selection requires further research

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