System and method for calculating TCID50 (Tissue Culture Infective Dose 50 percent)

Abstract

The invention belongs to the field of parameter calculation, and particularly relates to a system and a method for calculating the TCID50 (Tissue Culture Infective Dose 50 percent). The system comprises an input module, a primary calculation module, a judgment module, a secondary calculation module and a display module, wherein the input module is used for inputting measured experimental data; the primary calculation module is used for calculating and obtaining lesion percentage according to the experimental data; the judgment module is used for judging whether the lesion percentage which is equal to preset lesion percentage exists or not, using and sending dilution degree as the TCID50 to the display module and activating the display module if the lesion percentage exists, and activating the secondary calculation module if the lesion percentage does not exist; the secondary calculation module is used for calculating and obtaining TCID50 data according to the lesion percentage and the experimental data; the display module is used for displaying the data related to calculation. According to the method disclosed by the invention, the TCID50 calculation which is simple in calculation steps, small in workload, high in accuracy and high in efficiency is realized.

Description

technical field [0001] The invention belongs to the field of parameter calculation, in particular to a system and method for calculating the infection amount of half cell cultures. Background technique [0002] In laboratory operations, there are many methods for describing virus titers, mainly including physical methods and biological methods. The physical method is to measure the absorbance of virus particles at 260nm. The absorbance method is used to measure the virus titer of virus cultures in various laboratories. However, the disadvantage is that it cannot distinguish infectious and defective virus particles. In biological methods, plaque forming units (PFU) and half cell culture infectious dose (TCID 50 ) is the most widely used. PFU is the earliest standard method for virus titer determination. It is mainly used to measure the formation of virus lysis plaques in monolayer cell culture. Its disadvantage is mainly the poor repeatability of the experiment. The same e...

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Problems solved by technology

PFU is the earliest standard method for virus titer determination. It is mainly used to measure the formation of virus lysis plaques in monolayer cell culture. Its disadvantage is mainly the poor repeatability of the experiment. same experimental result

TCID 50 The existing method is to manually draw a table through manual experiments, fill in the table with the number of CPE, the total number of holes and the number of no CPE according to the experimental results, calculate the data of up to 20 grids according to the table, and use the corresponding data according to the calculated data. The formula to get the final result has cumbersome calculation steps, heavy workload, low accuracy, long time consumption, low efficiency, and the result is not saveable

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