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Bacillus subtilis for degrading aflatoxin b1 and its application

A technology of Bacillus subtilis and aflatoxin, applied in the field of Bacillus subtilis, can solve the problem that the degradation effect of high-concentration AFB1 needs to be improved

Active Publication Date: 2019-06-28
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, Bacillus subtilis ANSB060 and Bacillus subtilis ANSB324 strains only have a higher degradation rate (more than 80%) to lower concentrations of AFB1 (final concentration 0.5 μg / mL), and the degradation effect on high concentrations of AFB1 needs to be improved

Method used

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  • Bacillus subtilis for degrading aflatoxin b1 and its application
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  • Bacillus subtilis for degrading aflatoxin b1 and its application

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Embodiment 1

[0024] Bacillus subtilis S1 for degrading aflatoxin B1, deposit number: CCTCC NO: M 2016390, deposit date: July 12, 2016, depository unit: China Center for Type Culture Collection (CCTCC), deposit Address: Wuhan, China. Wuhan University (Wuhan University Collection Center, Luojia Mountain, Wuchang, Wuhan City, Hubei Province).

Embodiment 2

[0026] The application of Bacillus subtilis S1 (CCTCC NO: M 2016390) in degrading aflatoxin B1 includes: taking Bacillus subtilis S1 for fermentation and culturing, and the obtained fermentation liquid is used to degrade aflatoxin B1. Alternatively, the fermentation broth is centrifuged (to remove bacteria), and the active protein in the supernatant is separated, and the active protein is used to degrade aflatoxin B1.

Embodiment 3

[0028] The application of Bacillus subtilis S1 (CCTCC NO: M 2016390) in the preparation of biological feed additives for degrading aflatoxin B1 includes: taking Bacillus subtilis S1 for fermentation and culture, centrifuging after cultivation to obtain supernatant; The active protein is separated and mixed with auxiliary materials to obtain a biological feed additive.

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Abstract

The invention discloses a bacillus subtilis for degrading aflatoxin B1 and an application thereof, belonging to the field of biotechnology. The present invention uses coumarin as the only carbon source and energy source for primary screening, and uses the degradation rate of the strain to AFB1 as the indicator for secondary screening, and obtains a strain of Bacillus subtilis S1 (CCTCC NO:M 2016390) that can efficiently degrade AFB1. The degradation rate of the strain's fermentation broth to AFB1 reached 61.36%; after culturing with 10% inoculum, the degradation rate reached 80.26%; after the fermentation supernatant was concentrated 8 times, the degradation rate reached 85.65%. Studies have shown that the extracellular enzymes secreted by Bacillus subtilis S1 can degrade aflatoxin B1, so the fermentation broth of this strain or the active protein isolated from the fermentation broth of the strain can be used to degrade aflatoxin B1 or prepare a product that degrades aflatoxin B1. biological additives.

Description

technical field [0001] The invention relates to a bacillus subtilis for degrading aflatoxin B1, and also relates to the application of the bacillus subtilis, which belongs to the field of biotechnology. Background technique [0002] Aflatoxins (AFT) are mainly highly toxic secondary heterocyclic metabolites produced by various fungi such as Aspergillus flavus and Aspergillus parasiticus. Due to improper storage and transportation, grain, feed and food are prone to mildew and produce aflatoxin. Among the aflatoxins, AFB1 is the most toxic and has strong carcinogenic, teratogenic and immunosuppressive effects on humans and animals. Therefore, solving the problem of aflatoxin pollution in grain and feed is of great significance to improving food safety and ensuring animal health. [0003] At present, the removal methods of aflatoxin in grain and feed mainly include physical, chemical and biological methods. However, the traditional physical and chemical methods have many sho...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L5/20A23K10/18C12R1/125
CPCC12N1/205C12R2001/125
Inventor 王彦彬魏单平闫峰宾蒋瑞瑞李转见田亚东刘小军康相涛
Owner HENAN AGRICULTURAL UNIVERSITY
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