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Method for quickly, easily and conveniently measuring contents of proteins in fruiting bodies of clavicipitaceae fungus stromata

A technology of protein content and fungus, applied in the field of detection of edible and medicinal fungal fruiting bodies, can solve the problems of few samples, long time, environmental pollution, etc., and achieve the effects of simple operation, low cost, and low operator requirements.

Inactive Publication Date: 2017-10-17
SOUTH CHINA AGRI UNIV
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Problems solved by technology

[0004] According to literature review, the detection method of protein content in Myrogia mainly adopts the traditional physical and chemical analysis method. This analysis method samples few samples and takes a long time. During the analysis process, a large amount of waste acid and alkali are produced. The environment has certain pollution

Method used

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  • Method for quickly, easily and conveniently measuring contents of proteins in fruiting bodies of clavicipitaceae fungus stromata
  • Method for quickly, easily and conveniently measuring contents of proteins in fruiting bodies of clavicipitaceae fungus stromata
  • Method for quickly, easily and conveniently measuring contents of proteins in fruiting bodies of clavicipitaceae fungus stromata

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Embodiment 1

[0035] The specific implementation of each step is described in detail below.

[0036] ①The original spectrum of the fruiting body sample of Chimicronium:

[0037] The fruiting bodies of different varieties were cultivated in different culture methods, and at the same time, 55 samples of Myrogia from different origins and sources were collected on the market. Dry the sample at 55°C for 48 hours, then pulverize it with a universal pulverizer, and pass it through a 90-mesh sieve to obtain Cordyceps powder; turn on the near-infrared spectrometer at 25°C to preheat for 30 minutes, and take about 5g of Cordyceps powder in a diameter of 32mm and a thickness of 45mm In the rotating sample cup, the spectrum is collected by the diffuse reflection method of the integrating sphere, the spectrum collection range is 4000-10000cm-1, the number of scans is 32, and each sample is loaded repeatedly 3 times to obtain the original spectrum of the calibration set sample, and the original spectrum...

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Abstract

The invention discloses a method for quickly, easily and conveniently measuring the contents of proteins in fruiting bodies of clavicipitaceae fungus stromata. The method includes steps of collecting cordyceps powder samples, carrying out near infrared spectrum scanning on the samples and acquiring spectra to obtain original spectra of samples of calibration sets; preprocessing the original spectra of the samples of the calibration sets; carrying out principle component analysis on information data of the preprocessed original spectra of the samples of the calibration sets and extracting feature information data; chemically detecting the contents of proteins of samples of the fruiting bodies of the clavicipitaceae fungus stromata to obtain chemical measurement values; setting the chemical measurement values as calibration values, utilizing the feature information data as independent variables, utilizing the calibration values as dependent variables and building calibration models by the aid of partial least squares processes; carrying out internal cross-validation and external cross-validation on the calibration models and evaluating the reliability of the calibration models. The method has the advantages of high detection speed, multiple detection samples, low detection cost, accurate detection results and zero pollution.

Description

technical field [0001] The invention relates to the field of detection of edible and medicinal fungus fruiting bodies, in particular to a method for quickly and easily measuring the protein content in the fruiting bodies of myromycetes. Background technique [0002] Migonia is the subsea of ​​Ergotaceae fungi parasitic on the larvae of Batmothidae insects. It is a traditional precious nourishing edible and medicinal fungus. Antioxidant and other functions. It is rich in protein, polysaccharide, cordycepic acid, cordycepin and adenosine substances. In addition, it also contains vitamin B12, ergothioneine, saponins, various alkaloids, etc. [0003] According to literature reports: the content of each component of the fruiting bodies of Myrogia cultured by different varieties is quite different, and the content of its components is also affected by the type of culture medium, so the quality of different varieties, places of origin, and batches has relatively large differences...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/3563G01N21/359G01N31/00G01N31/16
CPCG01N21/3563G01N21/359G01N31/002G01N31/16
Inventor 郭丽琼李雄林俊芳郑倩望魏韬叶志伟
Owner SOUTH CHINA AGRI UNIV
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