Breeding method for enhancing salt tolerance ability domestication of potamogeton pectinatus

Abstract

The invention discloses a breeding method for enhancing salt tolerance ability domestication of potamogeton pectinatus. The method comprises the steps that potamogeton pectinatus seeds with a determined salt tolerance range are soaked, peeled and cleaned; a planting substrate with the total salt content the same as the salt tolerance upper limit value of the selected seeds is prepared, the seeds are planted in the planting substrate, a salt-containing aqueous solution with the salt content equal to that of the planting substrate is poured into the planting substrate, and the pH value of the substrate solution is adjusted to reach the alkali resistance growth extreme value; after the seeds germinate, the planting substrate is irrigated with a NaCl aqueous solution with the salt content 0.5 % higher than that the planting substrate solution every 7 days, Na2CO3 with the pH value 0.3 higher than that of the planting substrate solution, a NaHCO3 solution and 20 mg*L<-1> IAA aqueous solution are applied to the planting substrate, the steps are continuously repeated, experiment operation is stopped till growth physiological indexes of seedlings are not increased and the whole plants start to become yellow, then the seedlings transplanted to a field to be subjected to seedling hardening, and a new potamogeton pectinatus variety which is higher in salt tolerance in the salt-tolerance limit range and can adapt to different saline-alkali regions is obtained through experimental planting. By the adoption of the method, the plant salt tolerance domestication effect can be deeply developed.

Description

technical field [0001] The invention belongs to the technical field of salt tolerance of submerged plants, and in particular relates to a breeding method for acclimating the salt tolerance of submerged plants. Background technique [0002] The soil and water in coastal areas have high salinity and alkali content, low vegetation coverage, and water pollution brought about by rapid economic development, resulting in scarce local aquatic vegetation. It has become a priority to screen and study plants that can tolerate salt and alkali and have a certain purification function on water. It is imperative. Generally, submerged plants are suitable for growing in soil or water bodies with a pH of 5.5-7.0 and a salinity of 0-3.5‰, but when the salinity is greater than 6‰ and the pH is greater than 8.1, the salinity will cause ion poisoning to plants, causing osmotic stress, The balance of matter and energy is destroyed, and plants die because they cannot perform normal cell metabolism...

AI Technical Summary

Problems solved by technology

Grate eye vegetable ( Potamogeton pectinatus L. ) is a plant with a wide ecological range and strong stress resistance. It can be used as a submerged plant to restore polluted water bodies, as a native plant to compete with alien species, as a halophyte to green saline-alkali land, etc. It can improve the saline-alkali environment in saline-alkali land. During its growth cycle, the average soil pH is reduced from 8.22 to 7.79, and the average electrical conductivity is reduced by 55.3%. little research