Breeding method for enhancing salt tolerance ability domestication of potamogeton pectinatus
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A technology of ricochet eyes and salt tolerance, which is applied in the field of breeding of submerged plants for salt tolerance domestication, can solve the problems of little research and reduced electrical conductivity, and achieve an effect that is conducive to deepening
Inactive Publication Date: 2017-11-24
南京中科水治理股份有限公司
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Problems solved by technology
Grate eye vegetable ( Potamogeton pectinatus L. ) is a plant with a wide ecological range and strong stress resistance. It can be used as a submerged plant to restore polluted water bodies, as a native plant to compete with alien species, as a halophyte
Method used
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Embodiment 1
[0021] Embodiment 1: a kind of breeding method that strengthens the domestication of salt-tolerant ability of ricinosa, the method comprises the following steps:
[0022] 1. Collect ricinia oleracea seeds with good appearance and no attachments, and determine the salt tolerance range of the selected varieties (about 6‰);
[0023] 2. Soak the above-mentioned seeds in baking soda water with a mass concentration of 10% for 12 hours, rub off the colloidal body wrapped in the seed epidermis, wash, and then dry naturally in the shade to obtain clean castoria oleracea seeds;
[0024] 3. Use the saline-alkali subsoil at the bottom of the saline-alkali lake and ordinary sandy loam to prepare a planting base with a total salt content equivalent to the upper limit of the salt tolerance limit of the selected species; among them, the ordinary sandy loam has a sand content of 80%, and clay accounts for 80%. 20% soil;
[0025] 4. Sow castoria oleracea seeds in the prepared planting base, an...
Embodiment 2
[0031] Embodiment 2: a kind of breeding method that strengthens the domestication of salt-tolerant ability of ricinosa, the method comprises the following steps:
[0032] 1. Collect ricinia oleracea seeds with good appearance and no attachments, and determine the salt tolerance range of the selected varieties (about 6‰);
[0033] 2. Soak the seeds in baking soda water with a mass concentration of 10% for 10 hours, rub off the colloids wrapped in the seed epidermis, wash them, and dry them naturally in the shade to obtain clean castoria oleracea seeds;
[0034] 3. Use the saline-alkali subsoil at the bottom of the saline-alkali lake and ordinary sandy loam to prepare a planting base with a total salt content equivalent to the upper limit of the salt tolerance limit of the selected species; among them, the ordinary sandy loam has a sand content of 80%, and clay accounts for 80%. 20% soil;
[0035] 4. Sow castoria oleracea seeds in the prepared planting base, and at the same tim...
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Abstract
The invention discloses a breeding method for enhancing salt tolerance ability domestication of potamogeton pectinatus. The method comprises the steps that potamogeton pectinatus seeds with a determined salt tolerance range are soaked, peeled and cleaned; a planting substrate with the total salt content the same as the salt tolerance upper limit value of the selected seeds is prepared, the seeds are planted in the planting substrate, a salt-containing aqueous solution with the salt content equal to that of the planting substrate is poured into the planting substrate, and the pH value of the substrate solution is adjusted to reach the alkali resistance growth extreme value; after the seeds germinate, the planting substrate is irrigated with a NaCl aqueous solution with the salt content 0.5 % higher than that the planting substrate solution every 7 days, Na2CO3 with the pH value 0.3 higher than that of the planting substrate solution, a NaHCO3 solution and 20 mg*L<-1> IAA aqueous solution are applied to the planting substrate, the steps are continuously repeated, experiment operation is stopped till growth physiological indexes of seedlings are not increased and the whole plants start to become yellow, then the seedlings transplanted to a field to be subjected to seedling hardening, and a new potamogeton pectinatus variety which is higher in salt tolerance in the salt-tolerance limit range and can adapt to different saline-alkali regions is obtained through experimental planting. By the adoption of the method, the plant salt tolerance domestication effect can be deeply developed.
Description
technical field [0001] The invention belongs to the technical field of salt tolerance of submerged plants, and in particular relates to a breeding method for acclimating the salt tolerance of submerged plants. Background technique [0002] The soil and water in coastal areas have high salinity and alkali content, low vegetation coverage, and water pollution brought about by rapid economic development, resulting in scarce local aquatic vegetation. It has become a priority to screen and study plants that can tolerate salt and alkali and have a certain purification function on water. It is imperative. Generally, submerged plants are suitable for growing in soil or water bodies with a pH of 5.5-7.0 and a salinity of 0-3.5‰, but when the salinity is greater than 6‰ and the pH is greater than 8.1, the salinity will cause ion poisoning to plants, causing osmotic stress, The balance of matter and energy is destroyed, and plants die because they cannot perform normal cell metabolism...
Claims
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Application Information
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