Primers, method and kit for detecting expression amount of chicken FAM134B gene

Abstract

The invention relates to the field of bioengineering and particularly relates to primers, a method and a kit for detecting the expression amount of a chicken FAM134B gene. The primers are shown in the formulas of SEQ ID NO: 1 to 6. The method comprises extracting total RNAs from chicken tissue, carrying out reverse transcription to obtain cDNA and carrying out fluorescent quantitative PCR on specific primers of an amplification target gene and a reference gene. The kit is composed of the specific primers shown in the formulas of SEQ ID NO: 1 to 6, SYBR Premix Ex Taq II (Tli RNaseH Plus) (2x), ROX Reference Dye II (50x) and sterile water. The detection method is simple and fast, can measure the gene expression amount of the FAM134B gene in chicken and can study the expression pattern of the FAM134B gene in various tissues of the chicken.

Description

Technical field [0001] The invention relates to the field of bioengineering, in particular to a kit for detecting chicken FAM134B gene expression. Background technique [0002] Gene expression refers to the process in which cells transcribes and translates genetic information stored in DNA sequences into biologically active protein molecules during their life. The study of gene expression law can understand the expression of the target gene in different tissues and organs, and provide help for further analysis of the function of the gene. Although there are translation and post-translational processing in the process of gene expression, mRNA is the basic control point for transcription initiation in gene expression, so the determination of gene mRNA expression level can better reflect gene expression level and biological function Strong and weak. The previous transcriptome study of our group found that in two different Jinghai yellow chicken individuals with fast and slow growt...

AI Technical Summary

Problems solved by technology

Studies have found that the loss of function and mutation of the FAM134B gene can encode new Golgi proteins, which affect the distribution and modification of proteins and lipids by the Golgi, resulting in impaired nociception and autonomic neuropathy

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