A Novel Rapid Cell Stain and Its Application

Abstract

The invention provides a novel quick cell stain and application thereof. The cell stain can be an aqueous solution, and comprises solute toluidine blue and sodium sulfate. The method by applying the cell stain comprises the steps of directly contacting the cell stain with a to-be-stained cell or tissue for staining so as to complete cell staining. The cell stain has the advantages of quick staining and fading prevention of stained cells. Furthermore, the cell stain has the advantages of safety, environmental friendliness, simple composition, small dosage, low price, convenience in preparation, long storage period and the like. When the cell stain is used for staining a cell attached on a microcarrier, the staining effect of the cell stain is superior to that of conventional stain, and is represented as clear cell shading, but non-stained microcarrier. The cell stain is quicker than existing mast cell stain, can be used for staining living cells, and is more sensitive and clearer to particle staining.

Description

technical field [0001] The invention relates to a cell staining agent and its application, in particular to a fast cell staining agent and its application, and belongs to the field of cell staining. Background technique [0002] In basic research, it is often necessary to observe the details of cell morphology in experiments. The fine structure of cells can be displayed in different colors through staining, so that the cell images can be clearly presented under the light microscope. The staining method provided by the prior art has many steps and takes a long time, and most of them need to go through a series of procedures such as cell fixation. In order to observe the cell morphology in time and quickly, the rapid staining method is often used, but the current rapid staining method generally has the following defects: [0003] (1) Most of the reagents used in the fast dyes have potential pollution or harm to the environment or the human body. [0004] (2) The components of...

AI Technical Summary

Problems solved by technology

The staining method provided by the prior art has many steps and takes a long time, and most of them need to go through a series of procedures such as cell fixation. In order to observe the cell morphology in time and quickly, the rapid staining method is often used, but the current rapid staining method generally has the following defects:

[0003] (1) Most of the reagents used in fast stains have potential pollution or harm to the environment or human body

[0004] (2) The composition of the dye solution is complex and difficult to prepare. Most of them need to be prepared and used immediately, and cannot be stored for a long time

[0005] (3) The process of using the staining agent is cumbersome, the cells need to be fixed, and the staining process takes a long time

[0006] (4) The scope of application is narrow, and generally only has fast staining effect on specific types of cells

[0007] (5) The staining time is short, and after rapid staining, it will fade in a short time, which cannot meet the needs of long-term real-time observation of living cells

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