Cartridge for purifying PCR product

Abstract

The invention discloses a cartridge for purifying a PCR product. A through hole is formed in an upper cover of the cartridge, a magnetic sheath is arranged in the through hole, an annular bulge is arranged in the middle of the magnetic sheath, a spring is sheathed on the magnetic sheath on the lower part of the annular bulge, a metal bar is arranged in the magnetic sheath, a sphere with the diameter slightly smaller than the inner diameter of the magnetic sheath is arranged at the bottom of the metal bar, the upper end of the metal bar extends out of the upper cover, the lower end of the metalbar is inserted in the bottom of the magnetic sheath, and the metal bar can drive the magnetic sheath to move up and down; and a plurality of centrifugal pipes are arranged on a base. Under the action of an outside magnetic field, the metal bar can generate magnetism which can be completely eliminated after the outside magnetic field is removed. Through the up-down movement of the metal bar and the magnetic sheath, the grab and release of magnetic beads can be completed, the capture, washing and rinsing of nucleic acid can be realized through simple operation, and finally, the purified PCR product can be obtained, the influence of aerosol escape generated during the purification process of the PCR product on the environment can be avoided.

Description

technical field [0001] The invention relates to a device for extracting biological nucleic acid molecules, in particular to a cartridge used for preventing aerosol pollution during the purification process of PCR products. Background technique [0002] PCR products generally contain excess primers, Taq DNase, and dNTPs. The existence of these components will directly affect the subsequent enzyme digestion, dideoxy PCR sequencing reactions, etc., so it is necessary to remove them. for 10 13 copy / ml), which is far higher than the limit of PCR detection copy number, and in the process of purification, it is very easy to generate aerosol and escape into the environment, causing pollution to the environment, which is difficult to eliminate in a short period of time. A difficult problem is of great significance to molecular diagnostic work or research. Contents of the invention [0003] The technical problem to be solved by the present invention is to overcome the defect that ...

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is to overcome the defect that aerosol pollution is easily generated during the purification of existing PCR products, and to provide a cartridge for purification of PCR products

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