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Swollen cell virus vSOCS/vTK double-gene knock-out strain as well as preparation method and application thereof

A technology of mast cell virus and double genes, applied in the field of mast cell virus vSOCS/vTK double gene knockout strain and its preparation, can solve the problems of lack of immune protection from natural infection, difficult promotion and application, and short immunity period , to achieve the effect of convenient immunization method, excellent protection rate and high immunization rate

Active Publication Date: 2018-04-06
SUN YAT SEN UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, inactivated vaccines are not easy to carry out large-scale production in aquaculture due to short immunization period, large dosage, need for adjuvants, lack of immune protection against natural infection, and need to consume a lot of manpower and material resources. promotion and application

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  • Swollen cell virus vSOCS/vTK double-gene knock-out strain as well as preparation method and application thereof
  • Swollen cell virus vSOCS/vTK double-gene knock-out strain as well as preparation method and application thereof
  • Swollen cell virus vSOCS/vTK double-gene knock-out strain as well as preparation method and application thereof

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Embodiment Construction

[0071] The following description is a preferred embodiment of the present invention, it should be pointed out that for those skilled in the art, without departing from the principle of the present invention, some improvements and modifications can also be made, and these improvements and modifications are also considered Be the protection scope of the present invention.

[0072] The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings. The experimental method that does not indicate specific condition in the embodiment, generally according to routine condition. Unless otherwise specified in the examples of the present invention, all reagents and consumables used are commercially available.

[0073] The embodiment of the present invention provides a preparation method of a recombinant viral vaccine with deletion of swollen cell virus vSOCS and vTK genes, comprising the following steps:

[0074] 1. Construc...

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Abstract

The invention provides a swollen cell virus vSOCS / vTK double-gene knock-out strain as well as a preparation method and application thereof. The swollen cell virus vSOCS / vTK double-gene knock-out strain is an attenuated virus strain prepared by deleting a vSOCS gene and a vTK gene from a swollen cell virus stain. The invention further provides a swollen cell virus vSOCS / vTK double-gene knock-out strain carrying a selection marker as well as a preparation method and application thereof. The swollen cell virus vSOCS / vTK double-gene knock-out strain carrying the selection marker is an attenuated virus strain prepared by deleting the vSOCS gene and the vTK gene from a swollen cell virus, and inserting a first selection marker gene and a second selection marker gene into locations at which the genes are deleted through homologous recombination respectively. The swollen cell virus vSOCS / vTK double-gene deleted strain provided by the invention is a recombined gene engineered vaccine from whichthe vSOCS and vTK disease-causing genes are knocked out, is very weak in toxicity, does not cause diseases and death of immunized fishes, can achieve a remarkable immunizing effect by means of immersion immunization, does not need injection, and has great application value.

Description

technical field [0001] The invention relates to the field of prevention and control of aquaculture diseases, in particular to a double gene knockout strain of swollen cell virus vSOCS / vTK and its preparation method and application. Background technique [0002] Mandarin fish is one of the "Four Famous Freshwater Fishes" in my country, and it is a rare and high-quality economic fish with high economic value. Mandarin fish is popular in the market. While optimistic about the development prospects of mandarin fish aquaculture, the increasingly prominent disease problem has become a bottleneck restricting the development of mandarin fish aquaculture, seriously affecting the enthusiasm of farmers. Its typical example is the iridescent virus cell swelling virus disease of mandarin fish that broke out in our province in 1994, resulting in the mass death of cultured mandarin fish within a week. At present, the disease is still a serious threat to the development of mandarin fish aq...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/01C12N15/90C12N15/65A61K39/12A61P31/20C12R1/93
CPCA61K39/12A61K2039/5254C12N7/00C12N15/65C12N15/902C12N2710/00021C12N2710/00034
Inventor 何建国郭长军翁少萍林易凡
Owner SUN YAT SEN UNIV
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