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Rice High Photosynthetic Efficiency Gene and Its Application

A technology of photosynthesis and genetics, applied in the field of crop molecular genetics, can solve the problems of destroying target gene functions, error-prone, gene frameshift mutations, etc.

Active Publication Date: 2020-10-27
ZHEJIANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the repair process is prone to errors, resulting in insertion / deletion of bases in the repaired sequence, resulting in gene frameshift mutations and destroying the function of the target gene

Method used

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  • Rice High Photosynthetic Efficiency Gene and Its Application
  • Rice High Photosynthetic Efficiency Gene and Its Application
  • Rice High Photosynthetic Efficiency Gene and Its Application

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0016] The present invention is further described below in conjunction with specific embodiment, but protection scope of the present invention is not limited thereto:

[0017] Step 1, CRISPR / Cas9 vector construction of Os05g0462000 gene

[0018] According to the nucleotide sequence (SEQ ID NO:1) of the Os05g0462000 gene, two CRISPR / Cas9 edited target sequence sgRNAs (can be designed by the CRISPR Design program (http: / / crispr.mit.edu / )) were designed, The sequence is sgRNA-1: 5'-GAACAAGGAGGAGTGCATGG-3', sgRNA-2: 5'-GATGTTGGCATGCTTCTCCAGGG-3'; and the sequence of the sgRNA is synthesized in a biotechnology company (such as Shanghai Invitrogen).

[0019] Two corresponding vectors were respectively constructed using the CRISPR / Cas9 kit (Biogle, Cat#BGK03), and the method was according to the product instructions.

[0020] Step 2, rice genetic transformation constructed by CRISPR / Cas9 vector

[0021] The two CRISPR / Cas9 vectors constructed above were respectively transformed int...

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Abstract

The invention relates to a gene for high photosynthetic efficiency of paddy rice and application of the gene, and belongs to the field of molecular genetics of crops. Specifically speaking, by carrying out knockout mutation on an Os05g0462000 gene of a rice variety Nipponbare, 2 knockout mutants, namely a mutant-1 and a mutant-2 of the Os05g0462000 gene are obtained; the corresponding sequences ofthe mutant-1 and the mutant-2 are SEQ ID NO:2 and SED ID NO:3 respectively. The Os05g0462000 gene is knocked out, so that the chlorophyll content of paddy rice leaf blades can be increased and thereby the photosynthesis of the paddy rice is effectively improved; the gene has an important application value of breeding at high yield.

Description

technical field [0001] The invention relates to a gene for promoting rice photosynthetic efficiency and application thereof, and belongs to the field of crop molecular genetics. Background technique [0002] Rice is one of the most important food crops in the world. Photosynthesis, which uses light energy to convert carbon dioxide and water into organic matter, is an important factor in biomass synthesis during rice growth. Therefore, the identification of rice genes with high photoefficacy functions has important application prospects. [0003] CRISPR (clustered regularly interspaced short palindromic repeats) / Cas9 technology is a very effective tool in gene function identification. The technology generates a single-stranded guide RNA (sgRNA) to guide the Cas9 nuclease to perform double-strand breaks and repairs on specific target gene sequences in the target genome. However, the repair process is prone to errors, resulting in the insertion / deletion of bases in the repai...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29A01H5/12A01H6/46
CPCC07K14/415C12N15/8213C12N15/8269
Inventor 马伯军袁俊杰陈析丰傅小进顾志敏
Owner ZHEJIANG NORMAL UNIVERSITY
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