Method for rapid variation screening of different base sources of artemisia capillaris and artemisia scoparia through UPLC-MS-MS
A UPLC-MS-MS and Artemisia captiva technology, which is applied in the field of research on Chinese medicinal ingredients, can solve the problems of identification difficulties and omissions of the same Chinese medicinal materials, and achieve the effect of improving accuracy, reproducibility and large amount of information
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[0043] A UPLC-MS-MS method for quickly screening the difference between different genetic sources of Artemisia captivaris and Artemisia bismuth, comprising the following steps:
[0044](1) Collect the medicinal materials of Artemisia capillary and Artemisia chinensis to be differentiated, and divide different batches of Artemisia capillary and Artemisia spp. Prepare the corresponding standard decoction samples of Artemisia chinensis and Artemisia chinensis standard decoction samples, pulverize them, pass through a 60-mesh sieve, and store them in the dark at -20°C;
[0045] Wherein, the standard decoction samples of Artemisia chinensis and Artemisia chinensis are obtained by the following method:
[0046] Take the medicinal materials of Artemisia capricornus and Artemisia basilicum respectively, add 16 times the amount of water each, heat to 80°C with strong fire, keep warm at 80°C for 1 hour intermittently, filter with a 350-mesh sieve while it is hot, and cool the filtrate r...
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