30results about How to "Rapid Analytical Assay" patented technology

The invention provides a method for rapidly and nondestructively detecting astaxanthin in C.zofingiensis cells. The method comprises the following steps of (1) re-suspending algal cells collected from a known C.zofingiensis suspension sample to regulate the known C.zofingiensis suspension sample into heavy suspension with cell density of 6*10(5) to 2*10<6>CFU / mL by virtue of de-ionized water or a phosphate buffer solution, detecting the average fluorescence intensity of the heavy suspension in an FL1 channel or an FL2 channel by virtue of a flow cytometer, and performing linear regression on a log value of the content of astaxanthin in the known C.zofingiensis suspension sample and a log value of the corresponding measured average fluorescence intensity in the FL1 or FL2 channel to obtain a standard curve; (2) preparing a sample to be detected: re-suspending algal cells collected from C.zofingiensis suspension to be detected to regulate the C.zofingiensis suspension to be detected into algal cell heavy suspension to be detected with cell density of 6*10(5) to 2*10<6>CFU / mL by virtue of de-ionized water or a phosphate buffer solution; (3) detecting the average fluorescence intensity of the sample to be detected in the FL1 channel or the FL2 channel by virtue of the flow cytometer, and calculating the content of astaxanthin in the sample to be detected according to the standard curve obtained in step (1). The method has the characteristic of high detection efficiency.

The invention discloses a bone mineral density detection equipment and detection method based on a magnetic resonance T2 relaxation time spectrum. A bone mineral density is detected based on the magnetic resonance T2 relaxation time spectrum. The detection equipment comprises a computer control terminal, a one-board magnetic resonance controller unit, a signal amplification and switch control unit and a magnet unit. According to the bone mineral density detection equipment and detection method, water molecule magnetic resonance transverse relaxation deamplification is measured firstly, the corresponding T2 relaxation time spectrum is obtained through a mathematical inversion method, structural characteristics of pores among bone trabeculae are determined by analyzing all peak values on the T2 relaxation time spectrum, and biological bone mineral density data is obtained through a regression analysis data processing method finally. The bone mineral density detection equipment and detection method can be applied to identification of osteoporosis, the functions of rapidly and nondestructively analyzing and measuring the bone mineral density, evaluating the bone quality and the like are achieved, and the bone mineral density detection equipment and detection method have the advantages of being safe and harmless to organisms, simple in equipment structure, small in size, light in weight, high in measurement precision, easy and convenient to operate, high in repeatability of measurement method and the like.

The electrophoresis system comprises: a 3Dcapillary array micro-chip with multiple separation units, a negative feeding and separation device with micro vacuum pump as the negative pressure source, a high-voltage dc power, and a laser fluorescence detection system. This invention reduces cost greatly, and has well repeatability.

The invention discloses a method for rapid determination of vitamin B1 and vitamin C in a multivitamin tablet. The method comprises the steps of: (1) sample treatment: conducting dissolution, extraction, centrifugation, filtration and other pretreatment steps on a sample to obtain a filtrate; (2) sample analysis: employing an in situ spray ionization device to perform analysis, sucking the filtrate and methanol in a volume ratio of 1:1 and using the mixture to fill a drawn borosilicate glass capillary, inserting a metal microelectrode, placing the glass capillary at the front end of an ion mobility spectrometer sample introduction port, applying spray voltage to ionize the sample, letting the sample enter a drift tube, performing separation, and then conducting detection by a faraday cup detector. The method for rapid determination of vitamin B1 and vitamin C in the multivitamin tablet provided by the invention has the advantages of simple operation, low cost, fast analysis speed, good specificity and accurate quantitation, also because of small volume, mobility and strong maneuverability of the ion mobility spectrometer, the method is expected to achieve field rapid analysis and determination of samples, and has wide application prospects.

The invention discloses a method for rapid variation screening of different base sources of artemisia capillaris and artemisia scoparia through UPLC-MS-MS. The method comprises the following steps: 1)collecting a to-be-distinguished artemisia capillaris and artemisia scoparia medicinal materials, preparing a standard decoction sample; 2) introducing the sample in a UPLC-MS-MS system for analysisto obtain the corresponding mass spectrum data; 3) directly introducing the primary collected data in Compound Discoverer 2.1, and performing data analysis; 4) employing a dynamic program calculationmethod to correct chromatographic peak drift, aligning chromatographic peaks, using a proximal clustering method for registering a substance with approached retention time to a compound; and 5) usinga variance analysis to calculate the variation substance, and determining the variation of different base sources. The compound mass spectrum information is output to an original file, transition to other format cannot be required, the compound mass spectrum information can be directly introduced to Compound Discoverer 2.2 database to search the compound, the method can rapidly lock the variationcompound in the traditional Chinese medicinal material samples, and is suitable for rapid analysis and determination of the large-batch samples.

The invention relates to a portable powder compact stacking molding device and application thereof. The device comprises a base, a support arranged on the base, an upper pressing die capable of being arranged on the support in a reciprocating moving manner in the vertical direction, a feeding guiding mechanism used in cooperation with the upper pressing die, a sample pressing part located below the upper pressing side and opposite to the upper pressing die, and a digital display supporting mechanism arranged at the bottom of the sample pressing part. The upper pressing die can be arranged on the support through a screw transmission mechanism in the vertical reciprocating moving manner. The portable powder compact stacking molding device is used for stacking molding and sample manufacturing of to-be-detected soil powder based on an X-ray fluorescent spectral analysis method. Compared with the prior art, the portable powder compact stacking molding device has the beneficial effects that the structure is simple, use is convenient, the device is light and capable of being carried, manufactured samples cannot be contaminated and are consistent in compactness, and operability and accuracy for measuring the soil samples through an XRF method are greatly improved.

The invention provides a method for manufacturing a copper-bearing container steel plate casting blank through precise measuring of the copper content in a nickel-copper alloy. The above method for producing a copper-bearing container steel plate comprises the steps of molten iron pretreatment, converter smelting and LF furnace refinement. The step of converter smelting comprises the substeps thatthe copper content in the nickel-copper alloy is firstly measured, and then the nickel-copper alloy is added. In LF furnace refinement, inclusion modification calcium treatment is needed, and the standing time is larger than or equal to 7 min after refinement is finished. The substep of measuring the copper content in the nickel-copper alloy comprises the substeps that interference separation iscarried out, the copper content in filter liquor is measured, supplementary and correction of precipitation adsorption are carried out, and the measured copper content in the filter liquor and the copper content measured through supplementary and correction of precipitation adsorption are added; according to interference separation, iron interference elements are separated through ammonium hydroxide; and the copper content in test liquid is measured through the ICP spectrum to achieve supplementary and correction. The surface quality of the manufactured casting blank is good, and no crack is found during acid pickling of corners.

The invention provides a method for producing copper-containing container steel plates by using the accurate measurement of copper content in nickel-copper alloys. The above-mentioned method for producing copper-containing container steel plates includes: molten iron pretreatment, converter smelting, LF furnace refining, slab continuous casting, steel billet Heating, rough rolling, finish rolling and coiling; wherein, the converter smelting includes: first measuring the copper content in the nickel-copper alloy, and then adding the nickel-copper alloy; accurately controlling the copper content; The temperature is 1300±30°C; the determination of the copper content in the nickel-copper alloy includes the following: interference separation, determination of the copper content in the filtrate, correction of the precipitation adsorption, and the comparison of the copper content measured in the filtrate with the copper content determined by the correction of the precipitation adsorption The contents are added together; the interference separation is the separation of iron interference elements with ammonia water; correction is carried out by measuring the copper content in the test solution with ICP spectroscopy. The casting slabs and steel products produced by the invention have good surface quality, and no defects such as cracks, pockmarks, peeling, cracks and the like are found.

The invention discloses a method for rapid variation screening of different base sources of artemisia capillaris and artemisia scoparia through UPLC-MS-MS. The method comprises the following steps: 1)collecting a to-be-distinguished artemisia capillaris and artemisia scoparia medicinal materials, preparing a standard decoction sample; 2) introducing the sample in a UPLC-MS-MS system for analysisto obtain the corresponding mass spectrum data; 3) directly introducing the primary collected data in Compound Discoverer 2.1, and performing data analysis; 4) employing a dynamic program calculationmethod to correct chromatographic peak drift, aligning chromatographic peaks, using a proximal clustering method for registering a substance with approached retention time to a compound; and 5) usinga variance analysis to calculate the variation substance, and determining the variation of different base sources. The compound mass spectrum information is output to an original file, transition to other format cannot be required, the compound mass spectrum information can be directly introduced to Compound Discoverer 2.2 database to search the compound, the method can rapidly lock the variationcompound in the traditional Chinese medicinal material samples, and is suitable for rapid analysis and determination of the large-batch samples.

The invention discloses a device and method for measuring the calcium content of cigarette paper. The sample liquid, the background liquid and the air are sent into the first glass coil through the background liquid pump tube, the sample pump tube and the first air pump tube respectively; Send it into the dialyzer, discharge the upper waste liquid after dialysis, then send buffer and air to the lower dialysate through the buffer pump tube and the second air pump tube respectively; output to the second glass coil after mixing, and at the same time The pure water pump sends pure water into the second glass coil; after mixing, it sends it into the third glass coil, and at the same time sends the developer into the third glass coil; The reaction solution is sent to the fourth glass coil, mixed evenly and then sent to the flow cell for colorimetry, and the effluent is discharged through the waste liquid pump tube. On the premise of ensuring the accuracy, precision and stability of the method, the present invention starts with simplified pretreatment, improves the detection efficiency of calcium content in cigarette paper, and realizes the purpose of rapid detection of large batches of samples.

The embodiment of the present invention provides a method for measuring the copper content in the nickel-copper alloy. The method for measuring the copper content in the nickel-copper alloy includes the following steps: sample dissolution, interference separation, determination of copper content, correction of precipitation adsorption, and adjustment of copper content. Calculation; The interference separation is to separate the iron interference element with ammonia; The measurement of the copper content is to adopt the selective decomposition of the combined masking agent composed of thiourea, phenanthroline and ascorbic acid Cu-EDTA complex , and finally titrate the released ethylenediaminetetraacetic acid EDTA with zinc standard solution, thereby calculate the content of copper; the correction of the precipitation adsorption is to dissolve the precipitation with dilute hydrochloric acid to dissolve the copper into the test solution, and measure the copper content by using ICP spectrometry. The copper content in the solution was corrected. The method provided by the invention for measuring the copper content in the nickel-copper alloy can solve the problem of inaccurate detection results of the current copper content in the nickel-copper alloy, and control the relative standard deviation RSD within 0.5%.

The invention discloses a method for rapid determination of vitamin B1 and vitamin C in a multivitamin tablet. The method comprises the steps of: (1) sample treatment: conducting dissolution, extraction, centrifugation, filtration and other pretreatment steps on a sample to obtain a filtrate; (2) sample analysis: employing an in situ spray ionization device to perform analysis, sucking the filtrate and methanol in a volume ratio of 1:1 and using the mixture to fill a drawn borosilicate glass capillary, inserting a metal microelectrode, placing the glass capillary at the front end of an ion mobility spectrometer sample introduction port, applying spray voltage to ionize the sample, letting the sample enter a drift tube, performing separation, and then conducting detection by a faraday cup detector. The method for rapid determination of vitamin B1 and vitamin C in the multivitamin tablet provided by the invention has the advantages of simple operation, low cost, fast analysis speed, good specificity and accurate quantitation, also because of small volume, mobility and strong maneuverability of the ion mobility spectrometer, the method is expected to achieve field rapid analysis and determination of samples, and has wide application prospects.

The invention discloses a method for measuring p-hydroxybenzoate in cosmetics and relates to a method for measuring components in cosmetics. The method comprises the following steps: A, performing tested sample treatment, namely preparing the tested cosmetics into diluent; B, performing spectral measurement, namely B1, preparing a background spectrum set, B2, determining the standard spectrum of p-hydroxybenzoate, and B3, performing spectral measurement on the tested cosmetics; C, performing positive test and content measurement, namely C1, performing positive test, calculating an included angle between the merged matrix and the standard spectrum of p-hydroxybenzoate, when the included angle is larger than 0.15, the p-hydroxybenzoate is judged to be negative, and the spectrum of the tested cosmetics is recorded into the background spectrum database, and when the included angle is smaller than 0.1, the p-hydroxybenzoate is stored in the tested cosmetics, the sample is positive, and continuing, and C2 performing content measurement, namely deducing the standard spectrum of p-hydroxybenzoate from the spectrum of the tested cosmetics, thereby obtaining the actual content of the p-hydroxybenzoate. The method is high in analysis efficiency, low in operation strength, low in analysis cost, high in robustness and easy to popularize and use.

The invention relates to a portable powder compact stacking molding device and application thereof. The device comprises a base, a support arranged on the base, an upper pressing die capable of being arranged on the support in a reciprocating moving manner in the vertical direction, a feeding guiding mechanism used in cooperation with the upper pressing die, a sample pressing part located below the upper pressing side and opposite to the upper pressing die, and a digital display supporting mechanism arranged at the bottom of the sample pressing part. The upper pressing die can be arranged on the support through a screw transmission mechanism in the vertical reciprocating moving manner. The portable powder compact stacking molding device is used for stacking molding and sample manufacturing of to-be-detected soil powder based on an X-ray fluorescent spectral analysis method. Compared with the prior art, the portable powder compact stacking molding device has the beneficial effects that the structure is simple, use is convenient, the device is light and capable of being carried, manufactured samples cannot be contaminated and are consistent in compactness, and operability and accuracy for measuring the soil samples through an XRF method are greatly improved.

The invention discloses a method for rapidly screening the difference of rhizoma corydalis and vinegar rhizoma corydalis through a UPLC-MS-MS. The method includes the steps of firstly, collecting to-be-distinguished medicine materials including rhizoma corydalis and vinegar rhizoma corydalis to prepare standard decoction samples; secondly, putting the samples in the UPLC-MS-MS to be analyzed to obtain corresponding mass spectrometric data; thirdly, directly importing the original collected data to a Compound Discoverer 2.1 for data analysis; fourthly, correcting chromatographic peak drifting and aligning chromatographic peaks through a dynamic scheduling and calculating method, and registering substances close in retention time into compounds through a most neighbor clustering method; fifthly, calculating different substances between the samples through variance analysis to determine the difference. Compound spectrometric information is output as an original file, the original file canbe directly imported into a Compound Discoverer 2.1 library to search the compounds without being converted into other formats, the different compounds existing in the medicine material samples can be rapidly locked through the method, and the method is suitable for rapidly analyzing and measuring samples on a large scale.

The present invention provides a method for producing copper-containing container steel plate casting slabs by using the accurate measurement of copper content in nickel-copper alloys. The method for producing copper-containing container steel plates includes: molten iron pretreatment, converter smelting, and LF furnace refining; wherein, the Converter smelting includes: first measure the copper content in nickel-copper alloy, and then add nickel-copper alloy; LF furnace refining needs to carry out inclusion denatured calcium treatment, and the sedation time after refining is ≥ 7 minutes; the determination of copper content in nickel-copper alloy includes the following : Interference separation, determination of copper content in the filtrate, correction of precipitation adsorption, adding the copper content measured in the filtrate to the copper content measured by correction of precipitation adsorption; the interference separation is the separation of iron interference elements with ammonia water ; Corrected by measuring the copper content in the test solution with ICP spectroscopy. The surface quality of the slab produced by the invention is good, and no cracks are found in the pickling of the edges and corners.

A rapid determination method for parabens in cosmetics, relates to a method for determination of ingredients in cosmetics, comprising the following steps: A. processing of samples to be tested; B. establishment of a background spectrum database: B1. acquisition Spectral data of cosmetic samples; B2. Obtain spectral data of components to be tested; B3. Convert data format; B4. Obtain the spectral data of the background without the component to be tested; B5. Dimensionality reduction of the background database; C. Obtain the standard spectral database v of parabens; D. Spectral measurement of the cosmetic sample to be tested; E. To be tested Determination of parabens content in cosmetic samples. The invention has high analysis efficiency, low operation intensity, low analysis cost, no need to accumulate a background database without components to be measured, can reduce machine time and reagent loss, has good robustness, and is simple in method, and is very suitable for the analysis of large batches of samples of the same kind Rapid analytical assay.

The invention relates to a method for analyzing and measuring typical metal in a circuit board of discarded electrical equipment and relates to the technology for pre-treating the circuit board of discarded electrical equipment and detecting and analyzing the content of the typical metal element. The method comprises the following steps: collecting a sample of the circuit board part of discarded electrical equipment and then performing the operations of surface dressing, cleaning, and rough and fine grinding on the sample; adding a proper amount of digestion reagent into the prepared sample, digesting the prepared sample by using a microwave digestion device and then extracting metal matter from the discarded circuit board; and using an inductively coupled plasma emission spectrometer to analyze the nature and quantity of the digested sample. According to the method, the operations of sample grinding and digesting are simple, the reagent consumption is less, the analysis result is accurate and reliable, the detection concentration range is wide, the technical problem of difficultly pre-treating the circuit board sample is solved, and the method is suitable for quickly identifying and accurately measuring the typical metal elements in the circuit boards of various discarded electrical products (such as televisions, refrigerators, washing machines, computers, printers, and the like) and the typical metal elements in the corner wastes generated in the circuit board production process.

The invention provides a method for measuring the contents of various skin antibacterial agents in wet tissues with ASE-HPLC to easily, quickly and sensitively detecting various skin antibacterial agents in wet tissues simultaneously.According to the technical scheme, firstly, liquid ingredients in wet tissues are squeezed out, and liquid is collected; secondly, 1 ml of the liquid obtained in the first step is metered and put into a 10 mL extracting pool, static extraction is carried out on the sample with acetone for 3 min at 80 DEG C, extraction is repeated two times, extraction liquid is made to the constant volume of 20 mL with acetone after extraction is finished, 1 mL of liquid is sucked into a centrifugal pipe, swirling is carried out for 2 min, centrifuging is carried out for 3 min at 15000 r / min, and supernate is collected; 3 ul of superanate is taken and injected into a high performance liquid chromatograph for detection, and a chromatogram is measured.The method belongs to the technical field of chemical detection.

The invention relates to a portable powder tableting sample preparation device, which comprises: a base; a shell arranged on the base: an opening is provided at the bottom; a spiral transmission structure: installed on the base along the vertical direction and located in the shell; Sample mold: it is movably arranged on the base and can seal the opening of the shell. Compared with the prior art, the present invention can ensure the continuous feeding, analysis, and discharge of samples, and has the advantages of precise pressure control, replaceable analysis contact surface mold, simple structure, portability, and dual use in the laboratory and field.

The invention provides a method for rapidly and non-destructively detecting intracellular astaxanthin in chlorella, comprising the steps of: 1) resuspending algae cells collected from known samples of chlorella suspension with deionized water or phosphate buffer solution, Adjust to a resuspension with a cell density of 6×105~2×106 CFU / mL, and use a flow cytometer to detect the average fluorescence intensity of the algae cell resuspension in the FL1 channel or FL2 channel; the known sample of the chlorella suspension The logarithm of the astaxanthin content and the logarithm of the average fluorescence intensity value of the corresponding measured FL1 or FL2 channel are linearly regressed to obtain a standard curve; 2) prepare the sample to be tested - with deionized water or phosphate buffer resuspend the algae cells collected from the chlorella suspension to be tested, and adjust it to a resuspension of the algae cells to be tested with a cell density of 6×105~2×106 CFU / mL; 3) use a flow cytometer to detect Measure the average fluorescence intensity of the sample in the FL1 channel or FL2 channel, and calculate the astaxanthin content according to the standard curve in step 1). The method of the invention has the characteristics of high detection efficiency.