Method of quickly screening difference of dry sulphitation radix paeoniae alba and non-dry sulphitation radix paeoniae alba by UPLC-MS-MS

Abstract

The invention discloses a method of quickly screening difference of dry sulphitation radix paeoniae alba and non-dry sulphitation radix paeoniae alba by UPLC-MS-MS. The method comprises the followingsteps: (1) collecting medicinal materials of dry sulphitation radix paeoniae alba and non-dry sulphitation radix paeoniae alba to be differentiated to prepare a standard decoction sample; (2) feedingthe sample into an UPLC-MS-MS system for analysis to obtain mass spectrometric data; (3) directly importing originally collected data into Compound Discoverer 2.1 to analyze data; (4) correcting chromatographic peak drift by adopting a dynamic planning calculating method, aligning the chromatographic peaks, and registering matters, the retaining times of which are the closest, into a compound by means of a nearest neighbor clustering method; and (5) calculating different matters among samples by means of variance analysis and determining the difference between dry sulphitation radix paeoniae alba and non-dry sulphitation radix paeoniae alba. The compound mass spectrum information is output as an original file which does not need to be converted into other formats and can be directly imported into a Compound Discoverer 2.1 library to search for compounds. The method can quickly lock different compounds in traditional Chinese medicinal material samples and is suitable for quick analysisand determination of bulk samples.

Description

technical field [0001] The invention belongs to the technical field of research on components of traditional Chinese medicines, and in particular relates to a UPLC-MS-MS method for rapidly screening the difference between Radix Paeoniae Alba and non-sulfur-fumigated Radix Paeoniae Alba. Background technique [0002] Radix Paeoniae Alba is a liver-nourishing and blood-tonifying drug commonly used in traditional Chinese medicine prescriptions. The traditional processing method is that the roots of Paeoniae Alba are peeled and boiled in water, then smoked with sulfur and then dried. At present, the primary processing method of Radix Paeoniae Alba is mainly boiled to the heart and then dried in the sun. However, because it is not easy to dry in the sun after boiling, and it is easy to mold, so the drug farmers generally use sulfur fumigation, especially in the main production areas of Radix Paeoniae Alba, such as Bozhou, Anhui. 50% of the Radix Paeoniae Alba are fumigated with s...

AI Technical Summary

Problems solved by technology

However, there are a large number of false positives and false negatives in these methods, which leads to the omission of important substance information when using these methods for analysis, which leads to the inability of the screened out differential substances to effectively represent group differences, which is of great importance for determining different processed substances. Difficulties in identification of the same kind of Chinese herbal medicines

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