Method for rapidly screening difference of rhizoma corydalis and vinegar rhizoma corydalis through UPLC-MS-MS

Abstract

The invention discloses a method for rapidly screening the difference of rhizoma corydalis and vinegar rhizoma corydalis through a UPLC-MS-MS. The method includes the steps of firstly, collecting to-be-distinguished medicine materials including rhizoma corydalis and vinegar rhizoma corydalis to prepare standard decoction samples; secondly, putting the samples in the UPLC-MS-MS to be analyzed to obtain corresponding mass spectrometric data; thirdly, directly importing the original collected data to a Compound Discoverer 2.1 for data analysis; fourthly, correcting chromatographic peak drifting and aligning chromatographic peaks through a dynamic scheduling and calculating method, and registering substances close in retention time into compounds through a most neighbor clustering method; fifthly, calculating different substances between the samples through variance analysis to determine the difference. Compound spectrometric information is output as an original file, the original file canbe directly imported into a Compound Discoverer 2.1 library to search the compounds without being converted into other formats, the different compounds existing in the medicine material samples can be rapidly locked through the method, and the method is suitable for rapidly analyzing and measuring samples on a large scale.

Description

technical field [0001] The invention belongs to the technical field of research on components of traditional Chinese medicines, and in particular relates to a UPLC-MS-MS method for rapidly screening the difference between Corydalis Corydalis and Corydalis Corydalis Vinegar. Background technique [0002] Corydalis yanhusuo is the dry tuber of Corydalis yanhusuo W.T.Wang, a plant of the poppy family. Its nature and flavor are pungent, bitter, warm, and return to the liver and spleen meridians. It has the functions of invigorating blood circulation, promoting qi circulation, and relieving pain. It is used for chest pain, epigastric pain, chest obstruction and heart pain, amenorrhea dysmenorrhea, postpartum stasis, and swelling and pain. . Also known as Yuanhu and Xuanhu, together with Atractylodes macrocephala, white peony root, Fritillaria fritillaria, Chrysanthemum chrysanthemum, Radix Scrophulariae, Ophiopogon japonicus, and curcuma, they are also known as "Eight flavors of...

AI Technical Summary

Problems solved by technology

[0004] At present, the identification methods of Chinese medicinal materials mainly include HPLC, IR, NIR, TLC, etc. There are a large number of false positives and false negatives in these methods, resulting in the possibility of missing important material information when using these methods for analysis, which leads to The screened differential substances cannot effectively represent group differences, which brings difficulties in identifying the same kind of Chinese herbal medicines with different processing methods

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