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Ngatively pressurized sampling three-dimensional chip capillary array electrophoresis system

A capillary array and capillary electrophoresis technology, which is applied in the direction of material analysis, measuring device, and instrument by electromagnetic means, can solve the problems of complex structure, long sampling time, expensive equipment, etc., and achieves lower production cost and faster sampling speed. , the effect of convenient operation

Inactive Publication Date: 2008-10-22
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Overcome the shortcomings of the current microfluidic chip capillary electrophoresis sampling equipment that is expensive, complex in structure and long in sampling time

Method used

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  • Ngatively pressurized sampling three-dimensional chip capillary array electrophoresis system
  • Ngatively pressurized sampling three-dimensional chip capillary array electrophoresis system
  • Ngatively pressurized sampling three-dimensional chip capillary array electrophoresis system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The three-dimensional capillary array electrophoresis microchip 1 provided in the present invention is made of three-layer substrate, and the substrate material can be glass, quartz or high molecular polymer. Drill holes at the appropriate position of the upper substrate, as three-dimensional capillary array electrophoresis Microchip public buffer pool B T , public buffer waste pool BW T , each sample solution pool S 1 -S n The outlet and common sample waste reservoir SW TThe inlet. On the substrate in the middle layer, a channel network containing a large number of capillary electrophoresis separation units is processed, and each separation unit is composed of a separation swim lane and a sample injection channel. The inlets of these separation lanes pass through a common buffer channel B bus Connect together, and pass through the small hole at the corresponding position of the upper substrate and the common buffer pool B T Connected together; the outlet end of th...

Embodiment 2

[0026] Example 2: Parallel separation and determination of multiple samples using a three-dimensional six-lane capillary chip array electrophoresis system with negative pressure injection

[0027] Negative pressure injection three-dimensional six-lane capillary chip array electrophoresis system such as image 3 As shown, it consists of a three-dimensional six-lane glass capillary electrophoresis microchip 1, a high-voltage DC power supply 2, a negative pressure sampling device 3 and a laser-induced fluorescence detector 4. For the negative pressure sampling device 3, refer to the invention patent (patent application number: 200610048906.6) , is made up of miniature vacuum pump 3-1, vacuum bottle 3-2, electrical contact vacuum gauge 3-3, three-way solenoid valve 3-4, timer 3-5 and interface 3-6. The laser-induced fluorescence detector is composed of a charge-coupled detection device (4-1) of CCD for short, a color filter (4-2, 4-7), a laser (4-4) and a lens (4-3, 4-5, 4 -6) Co...

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Abstract

The electrophoresis system comprises: a 3Dcapillary array micro-chip with multiple separation units, a negative feeding and separation device with micro vacuum pump as the negative pressure source, a high-voltage dc power, and a laser fluorescence detection system. This invention reduces cost greatly, and has well repeatability.

Description

technical field [0001] The invention relates to a microfluidic chip capillary electrophoresis analysis technology, in particular to a microchip capillary array electrophoresis production and sampling technology. Background technique [0002] Microfluidic chip capillary electrophoresis technology has been used in DNA sequencing, separation and identification of DNA fragments, separation and determination of amino acids, peptides, proteins, and analysis of components in single cells. However, chip capillary electrophoresis with only one separation lane cannot achieve multiple parallel determinations, and it is difficult to meet the needs of large-scale sample separation and analysis such as gene sequencing, proteomics research, clinical diagnosis, and drug screening. The development of a chip capillary array electrophoresis analysis system can greatly improve work efficiency and reduce analysis costs through multiple parallel determinations. [0003] At present, in chip capil...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/453G01N21/64
Inventor 殷学锋张磊
Owner ZHEJIANG UNIV
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