Method for detecting content of 9 components of polygala tenuifolia through quantitative analysis of multi-components by single marker (QAMS)

Abstract

The invention relates to a method for detecting content of 9 components of polygala tenuifolia through quantitative analysis of multi-components by single marker (QAMS). The method particularly comprises the following steps: S1, preparing a test sample solution; S2, preparing a control product solution; S3, formulating a chromatographic condition; and S4, performing detection and calculation. In the step S4 of detection and calculation, easily available 3,6'-disinapoyl sucrose with high content, low cost and high stability serves as an internal standard substance, the content is measured, andsibiricose A5, sibiricose A6, glomeratose A, tenuifolioside A, tenuifolioside B, tenuifolioside C, sibiricaxanthone B and polygalaxanthone III relative correction factors are calculated, and the content of the 8 components is calculated by the relative correction factors, so that the aim of detecting the 9 components of the polygala tenuifolia through QAMS can be fulfilled rapidly and accurately;meanwhile, the quality of the polygala tenuifolia can be determined through the content of the 9 components of the polygala tenuifolia.

Description

technical field [0001] The invention relates to the technical field of detection of components of traditional Chinese medicines, in particular to a method for detecting the contents of nine components of polygala by a one-test and multiple-evaluation method. Background technique [0002] Polygala tenuifolia Wild is a traditional Chinese medicine, first recorded in "Shen Nong's Materia Medica" and recorded in successive editions of "Chinese Pharmacopoeia". It has a variety of biological activities and therapeutic effects. Phlegm, detumescence effect, its main active ingredients include saponins, xanthones and oligosaccharides, etc., wherein saponins are the main ingredients of Polygala expelling phlegm and antitussive activity, which can improve short-term memory function, and Polygala xanthones has anti Tumor, anti-inflammatory, antithrombotic and antibacterial activities, in addition, polygala total oligosaccharide ester components have antidepressant activity, it can be se...

AI Technical Summary

Problems solved by technology

[0003] At present, the mainstream standard of Chinese medicine quality testing in my country still uses the traditional single component index to characterize its quality, which is difficult to truly reflect the pleiotropic and holistic nature of Chinese medicine.

In the prior art, multiple chemical composition combined detection technology (SDMC) has been more and more used in the detection of the composition of medicinal materials, and to evaluate the quality of traditional Chinese medicine. Based on the conventional chemical fingerprint analysis, SDMS mainly has two methods , the first method is the widely used external standard method, which requires multiple standard products to analyze the various components in the medicinal materials. This method is more scientific and reasonable than only measuring one index component, but this method takes a long time , and factors such as expensive, scarce and high-purity standards limit their use in scientific research and supervision, and the accuracy of the test results obtained by this method depends to a large extent on the control of operating conditions; the second method Using one reference substance to simultaneously determine the contents of multiple chemical components, that is, the QAMS technique (QAMS) solves the problem of expensive and insufficient quality of multiple reference substances in controlling the quality of medicinal materials, and provides a new research approach for quality control of traditional Chinese medicine. The experimental cost and detection time are reduced, but the method needs to carry out adaptability research to determine the selection of internal standard, the concentration of the reference solution and the proportion of each component in the reference solution

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