Method for controlling quality of fingerprint of dried extract of ficus microcarpa

A quality control method and fingerprint technology, applied in the field of quality control, can solve problems such as the difficulty in quality control of Ficus microphylla quality evaluation preparations, and achieve the effects of good precision, strong operability, and strong specificity

Active Publication Date: 2018-06-26
GUILIN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there have been research reports on the HPLC fingerprint of Ficus microphylla, which can reflect the attribution of medicinal materials from the shape of the spectrum, but there are still many components in the HPLC fingerprint that are unknown, which brings new problems to the quality evaluation of Ficus microphylla and the quality control of its preparations. difficulty

Method used

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  • Method for controlling quality of fingerprint of dried extract of ficus microcarpa
  • Method for controlling quality of fingerprint of dried extract of ficus microcarpa
  • Method for controlling quality of fingerprint of dried extract of ficus microcarpa

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0118] A method for quality control of the fingerprint spectrum of dry extract of Ficus microphylla, comprising the following contents:

[0119] (1) Preparation of the test solution:

[0120] Add 0.2g of Ficus lobata dry extract extract, add 40ml of 10% acetonitrile, accurately weigh, extract by ultrasonic, the ultrasonic power is 250W, the frequency is 30kHz, the ultrasonic time is 60min, shake well, let cool, and make up the weight with 10% acetonitrile, Shake well, filter through a 0.22um microporous membrane, and take the subsequent filtrate as the test solution.

[0121] (2) Preparation of reference substance solution:

[0122] Accurately weigh gallic acid, protocatechuic acid, 5-dicaffeoylquinic acid, catechin, caffeic acid, chlorogenic acid, epicatechin, orientin, vitexin, isoorientin, Appropriate amount of rutin, placed in a 100ml measuring bottle, fixed to volume with 70% methanol, diluted to a certain number of times, and the mass concentrations were 0.001694, 0.00...

Embodiment 2

[0144] A method for quality control of the fingerprint spectrum of dry extract of Ficus microphylla, comprising the following contents:

[0145] (1) Preparation of the test solution:

[0146] Add 0.2g of Ficus lobata dry extract extract, add 40ml of 10% acetonitrile, weigh it accurately, extract by ultrasonic, the ultrasonic power is 250W, the rate of extraction is 50kHz, the ultrasonic time is 30min, shake well, let it cool, and make up the weight with 10% acetonitrile , Shake well, filter with 0.22um microporous membrane, and take the continued filtrate as the test solution.

[0147] (2) Preparation of reference substance solution:

[0148] Accurately weigh gallic acid, protocatechuic acid, 5-dicaffeoylquinic acid, catechin, caffeic acid, chlorogenic acid, epicatechin, orientin, vitexin, isoorientin, Appropriate amount of rutin, placed in a 100ml measuring bottle, fixed to volume with 70% methanol, diluted to a certain number of times, and the mass concentrations were 0.00...

Embodiment 3

[0170] A method for quality control of the fingerprint spectrum of dry extract of Ficus microphylla, comprising the following contents:

[0171] (1) Preparation of the test solution:

[0172] Add 0.2g of Ficus microphylla dry extract extract, add 40ml of 10% acetonitrile, accurately weigh, ultrasonically extract, the ultrasonic power is 250W, the frequency is 40kHz, the ultrasonic time is 45min, shake well, let cool, add weight with 10% acetonitrile, Shake well, filter through a 0.22um microporous membrane, and take the subsequent filtrate as the test solution.

[0173] (2) Preparation of reference substance solution:

[0174] Accurately weigh gallic acid, protocatechuic acid, 5-dicaffeoylquinic acid, catechin, caffeic acid, chlorogenic acid, epicatechin, orientin, vitexin, isoorientin, Appropriate amount of rutin, placed in a 100ml measuring bottle, fixed to volume with 70% methanol, diluted to a certain number of times, and the mass concentrations were 0.001694, 0.005081, ...

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Abstract

The invention discloses a method for controlling the quality of the fingerprint of dried extract of ficus microcarpa. The fingerprint is LC-MS fingerprint of dried extract of ficus microcarpa. Througha reference substance, most common peaks are identified and especially, high-content flavonoids are identified. The method comprises chromatographic condition selection, mass spectrometry condition selection, test solution preparation, reference substance solution preparation, standard fingerprint establishment, identification and detection. The fingerprint provides the reference for further study on the pharmacodynamic mechanism and quality control of dried extract of ficus microcarpa.

Description

technical field [0001] The invention relates to the field of quality control, in particular to a method for controlling the quality of the fingerprint of the dry extract of Ficus xiaoye. Background technique [0002] Ficus microcarpa L.f. is the dry leaf of Ficus microcarpa L.f., a plant of the family Moraceae, mainly produced in Guangdong and Guangxi. The dry extract of Ficus xiaoye is the extract of the dried leaves of Ficus xiaoye. It is the main component of Ketralin, which is recorded in the quality standards of the 2015 edition of the Chinese Pharmacopoeia, and is used to treat cough and asthma and chronic bronchitis. The current Chp2015 pharmacopoeia standard uses HPLC to determine the content of vitexin and isovitexin in the dry extract of Ficus xiaoye, but does not analyze the main components such as flavonoids in the dry extract of Ficus xiaoye. [0003] Traditional Chinese medicine fingerprints are integral, macroscopic and fuzzy, and can comprehensively and comp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/72
CPCG01N30/02G01N30/72
Inventor 徐勤马超美邓立东饶伟文石元英
Owner GUILIN MEDICAL UNIVERSITY
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