Biological sample preparation method and application thereof and qualitative and quantitative metabolite analysis method

A biological sample and quantitative analysis technology, applied in the chemical field, can solve the problems of huge differences in metabolite concentration, achieve the effect of ensuring extraction efficiency and improving detection efficiency

Inactive Publication Date: 2018-07-17
麦特绘谱生物科技(上海)有限公司
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Problems solved by technology

[0003] The concentration of metabolites produced by various pathways varies greatly, and their physical and chemical properties also vary widely, especially the metabolites produced by the intestinal bacteria of the human body (organism) in the organism (host), and the relationship between intestinal bacteria and the organism (host). ) co-metabolites, including short-chain fatty acids, amino acids, benzoyl and phenyl derivatives, indole derivatives, lipids, bile a

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  • Biological sample preparation method and application thereof and qualitative and quantitative metabolite analysis method
  • Biological sample preparation method and application thereof and qualitative and quantitative metabolite analysis method
  • Biological sample preparation method and application thereof and qualitative and quantitative metabolite analysis method

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Embodiment 1

[0037] Prepare biological samples:

[0038] Select 76 persons without identification information from the sample bank (NMMS), and take the corresponding feces of the above 76 persons as fecal biological samples, that is, there are 76 fecal biological samples, and the feces can be fresh feces or frozen. Dried feces, in this embodiment, freeze-dried feces are used as the fecal biological sample;

[0039] Cellular biological samples were prepared with E. coli BL 21 (purchased from Sigma-Aldrich, St. Louis, MO) E. coli cells for validation of the metabolites detected in the above biological samples from position 76.

[0040] Quality control samples: the mixture of each stool biological sample is used as a stool quality control sample; the mixture of each cell biological sample is used as a cell quality control sample.

[0041] In the following, a derivative biological sample is obtained by using auxiliary reagents and derivatization reagents for derivatization reaction, and the a...

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Abstract

The invention provides a biological sample pretreatment method for treating a biological sample to obtain a pretreatment sample before gas chromatography and mass spectrometer combined detection and analysis on a metabolite in the biological sample from a living organism, application thereof to metabolite detection, and a corresponding metabolite detection and analysis method. The biological sample pretreatment method comprises the following steps: 1, performing extracting treatment; 2, performing a derivative reaction: performing the derivative reaction on the extracted biological sample by using an auxiliary reagent and a derivatization reagent to obtain a derivative biological sample; 3, standardizing and removing impurities, wherein the auxiliary reagent at least comprises a strong alkaline solution and a low-temperature alcohol organic solvent; in the step 1, the strong alkaline solution is used first and then the low-temperature alcohol organic solvent is used for continuously extracting the biological sample.

Description

technical field [0001] The invention belongs to the field of chemistry, and in particular relates to a biological sample used for processing biological samples to obtain pre-treated samples before performing gas chromatography and mass spectrometry detection and analysis on metabolites in biological samples from living organisms The preparation method and its application in the qualitative and quantitative analysis of metabolites, and the corresponding qualitative and quantitative analysis methods of metabolites. Background technique [0002] There are a large number of metabolites produced by various pathways in organisms, some are produced by the metabolism of the organism itself, some are produced by the metabolism of the organism and the microbiota in the organism, and some are produced by the microbiota in the organism produced by its own metabolism. The metabolites produced by each pathway exert their respective functions and cooperate with each other to affect the ov...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/02G01N30/06G01N30/88
Inventor 贾伟苏明明赵琳静倪艳
Owner 麦特绘谱生物科技(上海)有限公司
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