Human papillomavirus quick detection method, liquid-phase chip and kit

A human papillomavirus and liquid phase chip technology, applied in liquid phase chips and kits, in the field of rapid detection of human papillomavirus, can solve the problem that HPV cannot be accurately typed, other HPV subtypes are easily missed, and HPV cannot be clearly identified. Infection type and other problems, to overcome the missed detection of latent infection, improve stability and distinguishability, and achieve the effect of simple operation

Active Publication Date: 2018-08-07
杜权
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current method is mainly for HPV6, 11, 16 and 18 infection, and it is easy to miss other HPV subtypes
At the same time, fluorescent quantitative PCR cannot achieve accurate typing of HPV, that is, the type of HPV infection cannot be determined

Method used

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  • Human papillomavirus quick detection method, liquid-phase chip and kit
  • Human papillomavirus quick detection method, liquid-phase chip and kit
  • Human papillomavirus quick detection method, liquid-phase chip and kit

Examples

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Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Preparation of liquid phase chip

[0037] 1. Design and synthesis of capture probe sequences

[0038]For HPV-18, HPV-16, HPV-31, HPV-33, HPV-35, HPV-39, HPV-45, HPV-51, HPV-52, HPV-56, HPV-58, HPV-59, HPV -68a, HPV-68b, HPV-82, HPV-26, HPV-53, HPV-66, HPV-70, HPV-73, HPV-6, HPV-11, HPV-42, HPV-43 a total of 24 types For other nucleic acid sequences, capture probes complementary to the sequences were designed and synthesized respectively, and the sequences of the capture probes are shown in SEQ ID NO: 1-24. The capture probe was synthesized, and a biotin group was coupled to its 5' end to enable it to be coupled to the streptavidin group on the surface of the agarose microsphere. The capture probe sequences are shown in Table 1:

[0039] Table 1 Nucleotide capture probes for different HPV types

[0040]

[0041]

[0042] Each of the 4 types of HPV viruses (4 capture probes in total) was used as a group for detection, and the grouping situation is as ...

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Abstract

The invention discloses a human papillomavirus quick detection method, a liquid-phase chip and a kit. The liquid-phase chip comprises microsphere carriers, labeled molecules and nucleic acid capture probes aiming at different HPV types, the microsphere carriers are connected with coupling groups, the labeled molecules are available for independent detection, each capture probe comprises a specificnucleotide sequence and at least one coupling group, and each specific nucleotide sequence and target nucleic acid molecules coded by different types of HPV viruses form complementary pairs. By adoption of the method, accurate quantitative marking of the microsphere carriers can be realized, and stability and distinguishability in marking of the same type of microsphere carriers are remarkably improved. On the basis of the chip, the invention further provides a HPV detection method and a kit, 24 common HPV types can be detected in one time, the defect of detection omission in application of serology and immunohistochemical methods can be overcome while HPV detection efficiency is greatly improved, and early detection of the HPV viruses is realized.

Description

technical field [0001] The invention belongs to the technical field of nucleic acid detection, and in particular relates to a rapid detection method for human papillomavirus, a liquid phase chip and a kit. Background technique [0002] Human papillomavirus (human papillomaviruses, HPV) infection is closely related to the occurrence and development of various human tumors, especially cervical cancer. Cervical cancer is one of the most common cancers in women, second only to breast cancer in incidence. There are approximately 400,000 new cases worldwide each year and more than 200,000 women die from the disease. In developing countries, due to the imperfection of cervical cancer screening, its incidence rate is 6 times that of developed countries. In recent years, young cervical cancer patients have an obvious upward trend, and the incidence is increasing at a rate of 2% to 3% per year, causing great harm to women's physical and mental health. Therefore, an important part o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6837C12Q1/70C40B40/06
CPCC12Q1/6837C12Q1/708C40B40/06
Inventor 杜权王华全
Owner 杜权
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