Human papillomavirus quick detection method, liquid-phase chip and kit
A human papillomavirus and liquid phase chip technology, applied in liquid phase chips and kits, in the field of rapid detection of human papillomavirus, can solve the problem that HPV cannot be accurately typed, other HPV subtypes are easily missed, and HPV cannot be clearly identified. Infection type and other problems, to overcome the missed detection of latent infection, improve stability and distinguishability, and achieve the effect of simple operation
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[0036] Example 1: Preparation of liquid phase chip
[0037] 1. Design and synthesis of capture probe sequences
[0038]For HPV-18, HPV-16, HPV-31, HPV-33, HPV-35, HPV-39, HPV-45, HPV-51, HPV-52, HPV-56, HPV-58, HPV-59, HPV -68a, HPV-68b, HPV-82, HPV-26, HPV-53, HPV-66, HPV-70, HPV-73, HPV-6, HPV-11, HPV-42, HPV-43 a total of 24 types For other nucleic acid sequences, capture probes complementary to the sequences were designed and synthesized respectively, and the sequences of the capture probes are shown in SEQ ID NO: 1-24. The capture probe was synthesized, and a biotin group was coupled to its 5' end to enable it to be coupled to the streptavidin group on the surface of the agarose microsphere. The capture probe sequences are shown in Table 1:
[0039] Table 1 Nucleotide capture probes for different HPV types
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[0042] Each of the 4 types of HPV viruses (4 capture probes in total) was used as a group for detection, and the grouping situation is as ...
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