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A rapid detection method, liquid phase chip and kit for human papillomavirus

A human papillomavirus and liquid-phase chip technology, which is applied in the field of rapid detection of human papillomavirus, liquid-phase chips and kits, can solve the problem that HPV cannot be accurately typed, other HPV subtypes are easily missed, and HPV cannot be clearly identified. Infection type, etc.

Active Publication Date: 2021-05-28
杜权
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current method is mainly for HPV6, 11, 16 and 18 infection, and it is easy to miss other HPV subtypes
At the same time, fluorescent quantitative PCR cannot achieve accurate typing of HPV, that is, the type of HPV infection cannot be determined

Method used

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  • A rapid detection method, liquid phase chip and kit for human papillomavirus
  • A rapid detection method, liquid phase chip and kit for human papillomavirus
  • A rapid detection method, liquid phase chip and kit for human papillomavirus

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Example 1: Preparation of liquid phase chip

[0037] 1. Design and synthesis of capture probe sequences

[0038]For HPV-18, HPV-16, HPV-31, HPV-33, HPV-35, HPV-39, HPV-45, HPV-51, HPV-52, HPV-56, HPV-58, HPV-59, HPV -68a, HPV-68b, HPV-82, HPV-26, HPV-53, HPV-66, HPV-70, HPV-73, HPV-6, HPV-11, HPV-42, HPV-43 a total of 24 types For other nucleic acid sequences, capture probes complementary to the sequences were designed and synthesized respectively, and the sequences of the capture probes are shown in SEQ ID NO: 1-24. The capture probe was synthesized, and a biotin group was coupled to its 5' end to enable it to be coupled to the streptavidin group on the surface of the agarose microsphere. The capture probe sequences are shown in Table 1:

[0039] Table 1 Nucleotide capture probes for different HPV types

[0040]

[0041]

[0042] Each of the 4 types of HPV viruses (4 capture probes in total) was used as a group for detection, and the grouping situation is as ...

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Abstract

The invention discloses a liquid-phase detection chip for detecting human papillomavirus, a detection method and a detection kit. The liquid-phase detection chip includes a microsphere carrier connected with a coupling group, an independently detectable labeling molecule, and nucleic acid capture probes for different HPV types. The capture probe contains a specific nucleic acid sequence and at least one The coupling group and the specific nucleic acid sequence can form a certain degree of complementary pairing with target nucleic acid molecules encoded by different types of HPV viruses. The method provided by the invention can realize accurate quantitative labeling of the microsphere carrier, and significantly improve the stability and distinguishability of labeling the same type of microsphere carrier. Based on the chip, the present invention further provides an HPV detection method and its kit, which can detect 24 common HPV types at one time, not only overcome the missed detection defects of serological and immunohistochemical methods, but also greatly improve the detection of HPV Efficiency, to achieve early detection of HPV virus.

Description

technical field [0001] The invention belongs to the technical field of nucleic acid detection, and in particular relates to a rapid detection method for human papillomavirus, a liquid phase chip and a kit. Background technique [0002] Human papillomavirus (human papillomaviruses, HPV) infection is closely related to the occurrence and development of various human tumors, especially cervical cancer. Cervical cancer is one of the most common cancers in women, second only to breast cancer in incidence. There are approximately 400,000 new cases worldwide each year and more than 200,000 women die from the disease. In developing countries, due to the imperfection of cervical cancer screening, its incidence rate is 6 times that of developed countries. In recent years, young cervical cancer patients have an obvious upward trend, and the incidence is increasing at a rate of 2% to 3% per year, causing great harm to women's physical and mental health. Therefore, an important part o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6837C12Q1/70C40B40/06
CPCC12Q1/6837C12Q1/708C40B40/06
Inventor 杜权王华全
Owner 杜权
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