Method for screening and identifying Xinjiang Dolang sheep breeding associated protein screening at different physiological periods

A technology of related proteins and identification methods, which is applied in the field of screening and identification of reproduction-related proteins in different physiological periods of Xinjiang Duolang sheep, can solve the problems of not applying screening and identification of ovary-related proteins

Inactive Publication Date: 2018-08-14
TARIM UNIV
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But at present, this technology has not been applied to the screening and identific

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  • Method for screening and identifying Xinjiang Dolang sheep breeding associated protein screening at different physiological periods
  • Method for screening and identifying Xinjiang Dolang sheep breeding associated protein screening at different physiological periods
  • Method for screening and identifying Xinjiang Dolang sheep breeding associated protein screening at different physiological periods

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Embodiment Construction

[0039] The technical solutions of the present invention will be further described in detail below in conjunction with specific embodiments.

[0040] A method for screening and identifying reproductive-related proteins in different physiological stages of Xinjiang Duolang sheep, comprising the following steps:

[0041] Step 1. Obtaining ovaries of Xinjiang Duolang sheep at different physiological stages:

[0042] Through estrus synchronization, estrus identification and pregnancy identification techniques, the interestrus, estrus and pregnancy Duolang sheep (raised at the Animal Experiment Station of Tarim University) were slaughtered and the ovaries were taken out immediately, and the tissue surface was washed with PBS buffer (PH7.4) , and then quickly put into liquid nitrogen for quick freezing, and brought back to the laboratory for storage at -80°C for later use.

[0043] Step 2, protein extraction:

[0044]Take 0.1 g of the ovaries of Duolang sheep during the estrus peri...

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Abstract

The invention discloses a method for screening and identifying Xinjiang Dolang sheep breeding associated protein screening at different physiological periods, which belongs to the technical field of biology. The method comprises the following steps: 1) obtaining ovaries of Xinjiang Dolang sheep at the different physiological periods; 2) extracting protein; 3) performing Bradford protein quantification and standard curve drafting; 4) labeling a sample and performing equivalent mixing; 5) performing HPLC grading on a C18 chromatographic column under high PH condition; 6) performing mass spectrometry; and 7) processing data. By labeling with an iTRAQ agent, a labeled peptide segment is subjected to high performance liquid chromatography HPLC separation and mass spectrum detection MS, and 4847proteins can be identified, wherein 470 proteins are capable of expressing obvious difference.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for screening and identifying reproductive-related proteins of Xinjiang Duolang sheep in different physiological periods. Background technique [0002] Duolang sheep has the excellent characteristic of estrus all the year round, can be in estrus all year round, and is less affected by the law of long and short sunshine, and can be bred according to "two births a year" or "three births every two years". However, most breeds of sheep are seasonally in estrus, which is greatly affected by the law of sunshine. Generally, they only go into estrus in summer and autumn, and can only reproduce once a year. Under the trend of large-scale and intensive farming, it is the pursuit of economic benefits to maximize the reproductive potential of livestock, but seasonal estrus obviously limits the play of reproductive potential of sheep. Judging from literature review and exper...

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Application Information

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IPC IPC(8): G01N30/02G01N27/62G01N33/68
CPCG01N27/62G01N30/02G01N33/6848G01N2560/00
Inventor 常卫华徐辉王娟红钱伟崔子龙
Owner TARIM UNIV
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