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Gas-chromatography measuring method for hydrogen content in blood

A technology of gas chromatography and determination method, which is applied in the field of gas chromatography determination of hydrogen content in blood, can solve the problems of cumbersome methods and low precision, and achieve the effect of simple and fast operation, high precision and avoiding waste

Inactive Publication Date: 2018-08-31
北京亿美汇丰网络科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a gas chromatographic determination method for hydrogen content in blood, which is used to solve the problems of cumbersome and low accuracy in existing methods for measuring hydrogen content in blood

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  • Gas-chromatography measuring method for hydrogen content in blood

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Embodiment 1

[0021] The present embodiment provides a gas chromatographic assay method for hydrogen content in blood, said method comprising the following steps: (1) adopting headspace method to prepare gas to be measured and background gas; (2) selecting gas chromatographic conditions: chromatographic column is WassonKC063 Capillary chromatographic column, column length 30m, pore diameter 530μm; valve box temperature 45°C; carrier gas flow rate 15mL / min; 7min; detector temperature 190°C. The chromatographic column carrier is N 2 ; The detector is a pulse discharge helium ionization detector GC-PDHID. (3) Using an automatic sampler to inject samples, the gas to be measured and the background gas are separated by the chromatographic column, and then enter the detector immediately, and the detector converts the sample components into electrical signals, which are amplified and recorded to form Gas chromatogram; (4) The peak value of hydrogen in the blood is the peak value of hydrogen in th...

Embodiment 2

[0027] The present embodiment provides a gas chromatographic assay method for hydrogen content in blood, said method comprising the following steps: (1) adopting headspace method to prepare gas to be measured and background gas; (2) selecting gas chromatographic conditions: chromatographic column is WassonKC063 Capillary chromatographic column, column length 30m, pore diameter 530μm; valve box temperature 40°C; carrier gas flow rate 10mL / min; 6min; detector temperature 180°C. The chromatographic column carrier is Ar; the detector is a pulse discharge helium ionization detector GC-PDHID. (3) Using an automatic sampler to inject samples, the gas to be measured and the background gas are separated by the chromatographic column, and then enter the detector immediately, and the detector converts the sample components into electrical signals, which are amplified and recorded to form Gas chromatogram; (4) The peak value of hydrogen in the blood is the peak value of hydrogen in the b...

Embodiment 3

[0030] The present embodiment provides a gas chromatographic assay method for hydrogen content in blood, said method comprising the following steps: (1) adopting headspace method to prepare gas to be measured and background gas; (2) selecting gas chromatographic conditions: chromatographic column is WassonKC063 Capillary chromatographic column, column length 30m, pore diameter 530μm; valve box temperature 50°C; carrier gas flow rate 20mL / min; 8min; detector temperature 200°C. The chromatographic column carrier is He; the detector is a pulse discharge helium ionization detector GC-PDHID. (3) Using an automatic sampler to inject samples, the gas to be measured and the background gas are separated by the chromatographic column, and then enter the detector immediately, and the detector converts the sample components into electrical signals, which are amplified and recorded to form Gas chromatogram; (4) The peak value of hydrogen in the blood is the peak value of hydrogen in the b...

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Abstract

The invention discloses a gas-chromatography measuring method for the hydrogen content in blood. The method includes the following steps that (1) to-be-measured gas and background gas are prepared with the headspace method; (2) gas chromatography conditions are selected; (3) sample feeding is carried out through an automatic sampler, the to-be-measured gas and the background gas are separated through a chromatographic column, and then immediately enter a detector, sample components are changed into electrical signals through the detector, and the electrical signals are amplified and recorded to form a gas chromatogram; (4) a peak value of hydrogen in the to-be-measured gas subtracts a peak value of hydrogen in the background gas to obtain a difference value, and the difference value is a peak value of hydrogen in the blood. The gas-chromatography measuring method is high in accuracy and easy and convenient to operate, and accurate detection of the hydrogen content in human blood can becompleted in half an hour.

Description

technical field [0001] The invention relates to the technical field of analysis, in particular to a gas chromatography determination method for hydrogen content in blood. Background technique [0002] Many recent studies in the field of medical biology have obtained a lot of evidence with various animal models and human experiments, suggesting that hydrogen has a very wide range of medical effects. There is a lot of evidence that hydrogen is very safe for the human body. Through a detailed investigation of the diet, work and rest, and physiological parameters of the long-lived elderly, it was finally found that the hydrogen content in the blood of the long-lived elderly is 3-4 times that of ordinary people, and it has nothing to do with environmental factors such as region, race, and eating habits. Therefore, This value can be used as one of the parameters for evaluating a person's health status. Therefore, it is very important to accurately measure the content of hydrogen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8804G01N2030/8822
Inventor 姜多
Owner 北京亿美汇丰网络科技有限公司
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