Gene markers for screening obsessive-compulsive disorder, schizophrenia, and depression and their applications

A technology for schizophrenia and obsessive-compulsive disorder, applied in the field of molecular biology, can solve the problems affecting the symptomatic treatment of mental diseases, expression differences, and poor diagnostic consistency.

Active Publication Date: 2021-05-07
SHANGHAI BIOMEDICAL LAB CO LTD
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the lack of pathological biopsy support of brain tissue, the diagnosis of mental illness mainly relies on psychoanalytic scales and the subjective judgment of doctors. The diagnosis of mental illness is often specious, and the diagnostic consistency is poor, which seriously affects the symptomatic treatment of mental illness.
[0003] Although the brain tissue is considered to be isolated from the blood to a high degree due to the existence of the blood-brain barrier, many scholars and experts are still working on the gene expression of blood cells, whether it is possible to find a substitute for "brain tissue cells" to treat corresponding diseases For example, the leukocytes cultured from patients with schizophrenia and their healthy family members have significantly different genes

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Gene markers for screening obsessive-compulsive disorder, schizophrenia, and depression and their applications
  • Gene markers for screening obsessive-compulsive disorder, schizophrenia, and depression and their applications
  • Gene markers for screening obsessive-compulsive disorder, schizophrenia, and depression and their applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Screening of characteristic gene markers for obsessive-compulsive disorder

[0023] Screening for trait genes for obsessive-compulsive disorder includes the following steps:

[0024] 1) Using the Affymetrix Gene Expression Using Affymetrix U133Plus 2.0 human whole gene expression profile chip to analyze the peripheral blood gene expression profile of 20 cases of obsessive-compulsive disorder patients, 20 cases of schizophrenia and 20 cases of depression patients, the above 60 samples were used as training set (Training set);

[0025] 2) Using the Affymetrix MAS5 method to normalize the gene expression profile chip detection results of each sample in the training set;

[0026] 3) Eliminate the probe set (Probeset) signals with excessively high and low expression signals in the total RNA, and select the probe set signals with moderate expression in all 60 samples of the training set for subsequent analysis;

[0027] 4) T-test method was used to select the diff...

Embodiment 2

[0033] Example 2 Using the screened characteristic gene markers of obsessive-compulsive disorder to screen and diagnose obsessive-compulsive disorder, schizophrenia, and depression

[0034] 1. Method steps:

[0035] 1) Collection of peripheral blood samples of samples to be tested: use BD PAXgeneRNA blood collection tubes from QIAGEN to collect peripheral blood samples from patients;

[0036]2) Extraction and purification of total RNA in the peripheral blood sample of the sample to be tested: use QIAGEN's PAXgeneBloodRNA Kit to extract and purify the total RNA in the peripheral blood, and use the Agilent BioAnalyzer 2100 microelectrophoresis analyzer to identify the integrity and yield of the extracted total RNA fragments;

[0037] 3) Reverse transcription reaction: use Life Techonolgy's High-Capacity cDNA ReverseTranscription kit reverse transcription kit, use total RNA as a template, and use Olig(dT) as a reverse transcription primer to perform a reverse transcription reacti...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a gene marker for screening obsessive-compulsive disorder, schizophrenia, and depression. The gene marker comprises: the characteristic gene sequence of obsessive-compulsive disorder shown in SEQ ID NO.1-SEQ ID NO.10. The invention also discloses the application of the above-mentioned gene markers in the preparation of products for screening and diagnosing obsessive-compulsive disorder, schizophrenia and depression. The gene marker of the present invention is used for the screening of obsessive-compulsive disorder, schizophrenia, and depression. It has high sensitivity and strong specificity, and the peripheral blood that is most easily collected clinically is used as the detection sample, which is convenient for detection and is suitable for obsessive-compulsive disorder. The differential diagnosis of schizophrenia and depression provides new objective molecular indicators for the detection of obsessive-compulsive disorder and other mental diseases, which will help to improve the problem that the current diagnosis of mental diseases relies too much on the subjective judgment of doctors, and has broad application prospects.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a gene marker for screening obsessive-compulsive disorder, schizophrenia and depression and its application. Background technique [0002] Mental illness is a group of nervous system diseases mainly manifested in behavioral and psychological disorders. Due to the lack of objective diagnostic indicators for most mental illnesses, the current diagnosis of mental illness is mainly through observing the patient's behavior and communicating with the patient, judging the patient's thinking, cognitive ability, and self-awareness, etc., to understand the condition. Because different doctors often have different understandings and understandings of diseases, resulting in poor consistency in the diagnosis of mental diseases, it is difficult to compare and interpret the research results reported by different researchers, which seriously affects the diagnosis and treatment of menta...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/158
Inventor 程昌明杨超段广东
Owner SHANGHAI BIOMEDICAL LAB CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap