Biosensor for electrochemically detecting phosphorylated beta-amyloid protein and construction method thereof

A technology of amyloid protein and biosensor, which is applied in the field of biosensing, can solve the problems of increased detection cost and reduced sensitivity, and achieves the effects of low detection limit, high sensitivity, and reduced interference of phosphate background
CN108535344AInactive Publication Date: 2018-09-14CENT SOUTH UNIV
5 Cites 2 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CENT SOUTH UNIV
Publication Date
2018-09-14
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a biosensor for electrochemically detecting phosphorylated beta-amyloid protein and a construction method thereof. A gold electrode is reacted with a mercaptocarboxylic acid compound for modification of carboxyl groups on the surface of the gold electrode; the carboxyl groups modified on the surface of the gold electrode are activated by an EDC / NHS mixed solution, the activated carboxyl groups and an Abeta antibody are subjected to amidation reaction, and after the amidation reaction is completed, ethanolamine is used to block unreacted sites on the surface of the goldelectrode, that is, the surface of the gold electrode is modified with the Abeta antibody; the gold electrode with the surface modified with the Abeta antibody is first subjected to antigen-antibody reaction with the phosphorylated beta-amyloid protein, and then is chelated with Ti<4+>-TiP-Cd<2+> signal probe nanoparticles to obtain the biosensor directly used for electrochemical detection. The biosensor can realize the quantitative detection of the phosphorylated beta-amyloid protein, the detection method is simple, the sensitivity is high, and the biosensor is favorable for popularization and use. .
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to a biosensor, in particular to a high-sensitivity electrochemical detection phosphorylated β-amyloid biosensor and a construction method thereof, which belong to the technical field of biosensing. Background technique

[0002] Alzheimer's disease has become one of the major diseases threatening human health in the 21st century. It is a neurodegenerative disease characterized by progressive decline in cognitive ability, irreversible harmful impairment and observable memory impairment. . Amyloid β-protein (Aβ) is the main protein component of amyloid plaques present in the brains of Alzheimer's disease (AD) patients, and the formation of amyloid plaques is associated with the gradual accumulation of amyloid β-protein . While phosphorylation modifications are particularly abundant in amyloid β-protein aggregates, studies have shown that extracellular Aβ is phosphorylated in secreted variants of protein kinases. Phosphorylation o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More