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Method for analyzing Maca symbolic metabolite based on liquid chromatography-mass spectrometry metabonomics

A metabolite and metabolomics technology, which is applied in the field of analysis of Maca's landmark metabolites based on liquid chromatography-mass spectrometry metabolomics, which can solve problems such as differences in content and achieve the effect of simple operation.

Active Publication Date: 2018-09-14
YANTAI NEW ERA HEALTH IND
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The HPLC spectrum shows that all 15 batches of maca contain these three glucosinolates, but the contents are different

Method used

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  • Method for analyzing Maca symbolic metabolite based on liquid chromatography-mass spectrometry metabonomics
  • Method for analyzing Maca symbolic metabolite based on liquid chromatography-mass spectrometry metabonomics
  • Method for analyzing Maca symbolic metabolite based on liquid chromatography-mass spectrometry metabonomics

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Embodiment 1

[0060] A method for analyzing maca marker metabolites based on liquid mass spectrometry metabolomics, the steps are as follows:

[0061] (1) Preparation of maca extract

[0062] Weigh 1g of each powder sample of 20 different phenotypes of maca, add 20mL of methanol to mix, respectively, after ultrasonic extraction for 20min, centrifuge at 3900rpm for 20min, and the obtained supernatant passes through a filter membrane with a pore size of 0.22μm to obtain the maca extract. liquid;

[0063] (2) Maca extract pretreatment

[0064] The Maca extract in step (1) is diluted 20 times with methanol, and the diluted Maca extract passes through a filter membrane with a pore size of 0.22 μm to obtain a filtrate;

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Abstract

The invention relates to a method for analyzing a Maca symbolic metabolite based on a liquid chromatography-mass spectrometry metabonomics. The method includes the steps: extracting the Maca metabolite by the aid of methyl alcohol, performing UHPLC-Q Exactive Orbitrap LC-MS analysis after preprocessing, processing data by the aid of a Compound Discover, leading the data in SIMCA14.0 software, analyzing the data by the aid of a principal component analysis (PCA) method, a partial least squares discriminant analysis (PLS-DA) method and an orthogonal-partial least squares discriminant analysis (OPLS-DA) method, and searching differential metabolites; inspecting effectiveness of PLS-DA model establishment by the aid of a substitution permutation inspection method. Results show that model establishment is effective. The analysis method is simple to operate, and Maca metabolites with different phenotypes are searched, so that the analysis method is used for distinguishing and identifying the Maca metabolites with different phenotypes.

Description

technical field [0001] The invention belongs to the technical field of detection and analysis, and in particular relates to a method for analyzing maca marker metabolites based on liquid mass spectrometry metabolomics. Background technique [0002] Maca (Lepidium meyenii Walp.), a plant of the genus Lepidium of the Cruciferae (Cruciferae), is native to the South American Andes at an altitude of 3,500 to 4,500 meters. It has been cultivated for more than 2,000 years and has edible and medicinal value. . Since the 1990s, the United States, Japan, Germany and many other countries have tried to introduce and cultivate Maca. In 2002, Maca was introduced into China. Over the past ten years, this plant has been successfully introduced and cultivated in many regions of China, such as Yunnan, Xinjiang, Jilin and Sichuan. [0003] The nutritional content of Maca is reasonable and various, mainly including: protein, various amino acids, fatty acids, cellulose, sugars, calcium, zinc,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 杨长军耿越王桐刘梅徐宏楠梁晓庆仲米存
Owner YANTAI NEW ERA HEALTH IND
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