Culture medium for screening and identifying aflatoxin B1 degrading bacteria and screening and identifying method

Abstract

The invention belongs to the technical field of microbial strain screening, and in particular discloses a culture medium for screening and identifying aflatoxin B1 degrading bacteria and a screening and identifying method. The screening and identifying method comprises two steps, namely cultivating and identifying, wherein the step of cultivating is implemented as follows: with coumarin as a unique carbon source, conducting preliminary screening and purifying so as to obtain a preliminary screening strain, and then degrading AFB1 with the addition of an AFB1 standard product; and the step of identifying is implemented as follows: identifying whether soil contains a strain for degrading aflatoxin B1 or not via HPLC. The method, which takes the coumarin as the unique carbon source, is free from pollution on raw materials and is highly specific; it can effectively, simply and accurately confirm whether the strain for degrading the aflatoxin B1 exists in the soil or not, so that screeningcost is reduced; and meanwhile, the strain, which is screened out, can be used for effectively removing the aflatoxin B1, and the regeneration of toxins can be prevented; therefore, an efficient and safe toxin removal method is provided.

Description

technical field [0001] The invention belongs to the technical field of microbial strain screening, and relates to a medium for screening and testing aflatoxin B1-degrading bacteria and a screening testing method. Background technique [0002] Aflatoxin is a class of secondary metabolites mainly produced by fungi such as Aspergillus flavus, Aspergillus parasiticus, Aspergillus fumiformis, and Aspergillus luteus. This toxoid is a structural analogue composed of a difuran ring and coumarin, and has strong liver toxicity. , mutagenicity, carcinogenicity and teratogenicity. The scope of aflatoxin contamination is relatively broad, such as peanuts, corn, dried fruits, milk and other foods, because of its strong toxicity, it has attracted more and more attention. my country is a big country in peanut export trade, and excessive aflatoxin is the main problem affecting my country's peanut export. More than twenty types of aflatoxins have been identified, of which aflatoxin B 1 (AF...

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Problems solved by technology

[0003] Removal of AFB at home and abroad 1 The methods mainly include physical methods such as irradiation, heat treatment, and adsorbent detoxification, and chemical methods such as strong alkali, strong acid, and oxidant treatment, but these physical and chemical methods are not only high in cost and low in efficiency, but some methods are also accompanied by toxic substances.

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