High-density lipoprotein cholesterol detection kit

Abstract

The invention discloses a high-density lipoprotein cholesterol detection kit which consists of a first reagent and a second reagent, wherein the volume of the first reagent is 3 times of that of the second reagent; the kit comprises a surfactant, cholesterol esterase, cholesterol oxidase, peroxidase, absolute ethyl alcohol and a thiophosphoric reagent. In the first reagent, non-high-density lipoprotein cholesterol in a serum sample is in reaction with the cholesterol esterase, the cholesterol oxidase and the peroxidase under the action of the surfactant, thereby being eliminated; in the secondreagent, the rest serum with high-density lipoprotein cholesterol is extracted through the absolute ethyl alcohol, proteins in the serum are denaturated, the solubility is degraded, then precipitateis generated, cholesterol is dissolved in the ethanol, and under the action of the thiophosphoric reagent, the cholesterol is in reaction with the thiophosphoric reagent, and then the content of the cholesterol is detected. Test results show that the kit disclosed by the invention is wide in linear range, high in accuracy and precision and in addition good in interference resistance.

Description

technical field [0001] The invention relates to the field of biochemical detection, in particular to a high-density lipoprotein cholesterol detection kit. Background technique [0002] Cholesterol and other lipids cooperate with apoproteins in serum to form lipoproteins. According to different physical properties, lipoproteins are divided into chylomicrons (CM), very low-density lipoprotein (VLDL), low-density lipoprotein (LDL), and high-density lipoprotein (LDL). Density lipoprotein (HDL) and more. High-density lipoprotein (HDL) receives cholesterol from various tissues including the arterial wall, transports cholesterol into the liver through direct reverse transport or indirect reverse transport, and then clears it out of the blood. Epidemiological and clinical studies have shown that high-density lipoprotein cholesterol (HDL-C) is negatively correlated with the incidence of atherosclerotic coronary heart disease, and high-density lipoprotein cholesterol (HDL-C) is a pre...

AI Technical Summary

Problems solved by technology

[0009] The polyethylene glycol (PEG) modification method needs to modify the enzyme, and the cost is high; the key to the removal method and the selective inhibition method is the choice of surfactant, but the use of surfactant is more difficult, and the masking of non-high-density lipoprotein Incomplete inhibition and incomplete release of high-density lipoprotein (HDL)

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