A simplified seed production method of rapeseed hybrid f1 seeds
A seed and rapeseed technology, which is applied in the field of simplified rapeseed hybrid F1 seed production, can solve the problems of high labor cost, time-consuming and laborious, and decreased purity of F1 seeds, and achieve labor cost savings, simple breeding procedures, and improved seed purity. Effect
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Embodiment 1
[0019] Example 1: Breeding of Polyma cytoplasmic male sterile restorer line 7-5STR with self-incompatibility and resistance to sulfonylurea herbicides due to mutation of acetolactate synthase gene
[0020] (1) Use Huayouza 12 restorer line 7-5 as the female parent (genotype BnS-7BnS-7) and a self-incompatible individual plant isolated from the F2 offspring of Huayouza 95 (genotype F1 was obtained by hybridizing to BnS-1300 (BnS-1300).
[0021] (2) Using the above-mentioned F1 as the female parent to backcross the restorer line 7-5 of Huayouza No. 12 to obtain BC1.
[0022] (3) Use the DNA molecular marker linked to the self-incompatibility gene BnS-1300 to perform DNA molecular detection on the above-mentioned BC1 population individual plants, and screen out the heterozygous genotype (BnS-1300BnS) at the self-incompatibility gene locus -7) single plant.
[0023] (4) Using Huayouza No. 12 restorer line 7-5 (genotype BnaAHAS3_BnaAHAS3) as the female parent to cross with 655 (g...
Embodiment 2
[0036] Example 2: Rapeseed Polyma Cytoplasmic Male Sterile Hybrid Seed Production
[0037] The method of the present invention comprises the use of the Polyma cytoplasmic male sterile line 616A and the restorer line 7-5STR which has both self-incompatibility and resistance to sulfonylurea herbicides due to the mutation of the acetolactate synthase gene to produce Hybrid F1 seed, the specific steps of its hybrid seed production method include:
[0038] (1) Mix 616A and 7-5STR seeds evenly at a ratio of 80%:20%.
[0039] (2) Under the isolation condition of the artificial net room, the above-mentioned uniformly mixed seeds are planted with a row spacing of 20 cm and a plant spacing of 10 cm.
[0040] (3) Spray Huashaling WP2 in the early stage of budding of rapeseed (specific operation process: first step, 5 grams of Huashaling WP2 wettable powder + 1000ml water to make mother liquor; second step, take 50ml of mother liquor + 15000ml water to make dilution solution; in the thi...
Embodiment 3
[0042] Example 3: Rapeseed Polyma Cytoplasmic Male Sterile Hybrid Seed Production
[0043] (1) Mix 616A and 7-5STR seeds evenly at a ratio of 80%:20%.
[0044] (2) Under the isolation condition of the artificial net room, the above-mentioned uniformly mixed seeds were planted at a density of 25,000 plants / mu.
[0045] (3) Spray Huashaling WP2 in the early stage of budding of rapeseed (specific operation process: first step, 5 grams of Huashaling WP2 wettable powder + 1000ml water to make mother liquor; second step, take 50ml of mother liquor + 15000ml water to make dilution solution; in the third step, spray the diluent directly and evenly on the rape leaves). After 15 days, choose a sunny day and spray again.
[0046] (4) The sterile line and the restorer line were mixed and harvested when the seeds were mature, and statistics showed that the hybrid purity was 94.8%.
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