Serum-free culturing system inducing differentiation of pluripotent stem cells into motor neuron cells

Abstract

The invention belongs to the technical fields of stem cell biology and cell culturing, and provides a serum-free culturing system inducing differentiation of pluripotent stem cells into motor neuron cells. The serum-free culturing system specially comprises a serum-free differentiation basic culture medium and an additive for promoting the differentiation of the motor neuron cells, wherein necessary components of the serum-free differentiation basic culture medium comprise a DMEM / F12 culture medium, a human serum albumin, L-glutamine, D-glucose, vitamin C, N2 / B27 cell culture additive and unnecessary amino acid. The additive for promoting the differentiation of the motor neuron cells specially comprises SB431542, dermomorphin, fibroblast growth factors-2, retinoic acid, BDNF, GDNF, CTNF, DAPT, Smoothened agonist, and cytarabine. The culture medium is adopted to help improve the differentiation of the motor neuron cells in a cell mixing system, the differentiation of other types of cells is reduced, and finally, the motor neuron cells with high purity can be obtained.

Description

technical field [0001] The invention belongs to the technical fields of stem cell biology and cell culture, and specifically relates to a serum-free culture system for promoting the differentiation of induced pluripotent stem cells into motor nerve cells. Background technique [0002] Neurodegenerative diseases are a kind of diseases with slow onset, progressive development and poor prognosis. So far, there is no effective cure method. Common clinical neurodegenerative diseases include Parkinson's disease, amyotrophic lateral sclerosis, spinal muscular atrophy, and Alzheimer's disease. Although these diseases have different etiologies, they all have different parts of the central nervous system and different degrees of neuron loss and dysfunction in pathology. Replacing lost neurons and restoring their function is a new approach to treating these diseases. Studies in recent years have found that stem cells cultured in vitro can not only proliferate and differentiate, but a...

AI Technical Summary

Problems solved by technology

[0004] Most of the medium for pluripotent stem cell culture and differentiation into nerve cells contains fetal bovine serum components. The components of serum are unknown and complex. Due to the large differences in batches of serum products from different manufacturers, the repeatability of the experiment is extremely low.

In addition, serum may also carry pathogens and other pathogenic factors, which greatly reduces the application value and safety of differentiated nerve cells in drug screening testing or cell therapy.

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