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Application of saa1 detection agent in preparation of kit for diagnosing Henoch-Schonlein purpura nephritis

A technology for purpuric nephritis and allergy, which is applied in the field of medical diagnosis to achieve high sensitivity and specificity

Active Publication Date: 2021-09-17
ZHEJIANG PROVINCIAL PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

No significant relationship between SAA1 and Henoch-Schonlein Purpura Nephritis has been found in the prior art

Method used

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  • Application of saa1 detection agent in preparation of kit for diagnosing Henoch-Schonlein purpura nephritis
  • Application of saa1 detection agent in preparation of kit for diagnosing Henoch-Schonlein purpura nephritis
  • Application of saa1 detection agent in preparation of kit for diagnosing Henoch-Schonlein purpura nephritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 iTRAQ screening differential proteins

[0029] Part I. Screening of urinary molecular markers of renal injury

[0030] 1. Main experimental materials:

[0031] Urine of patients with renal impairment (diabetic AKI patients, AKI+CKD patients, diabetic CKD patients, non-diabetic IgA CKD patients, non-diabetic non-IgA CKD patients, 50 cases each); healthy people (50 cases); iTRAQ kit.

[0032] 2. Experimental setup:

[0033] 2.1 Early detection and inclusion criteria for patients with renal disease

[0034] 2.1.1 AKI inclusion criteria (clinical diagnosis)

[0035] According to the following two criteria, as the inclusion criteria for early AKI (AKI stage I): the blood creatinine content increased by 26.2 μmol / L or increased to 1.5-1.9 times the baseline value within 48 hours; the urine output was less than 6 hours continuously (no more than 12 hours) 0.5ml / Kg / h.

[0036] 2.1.2 AKI+CKD (CKD-based AKI patients, that is, CKD complicated with AKI) inclusion cri...

Embodiment 2

[0081] Example 2 Results related to SAA1

[0082] According to the protein expression abundance, the protein fold difference is greater than 1.2 (up-regulation) or less than 0.83 (down-regulation) and p When <0.05, it was screened as a differential protein. The differential proteins screened out in each group were sorted according to the multiple of difference, and the top 10 differential proteins in each group were selected.

[0083] Among them, SAA1 protein was abnormally expressed in the urine of AKI patients with diabetes mellitus, and the up-regulation times were more than 2.4 times, but there was no significant change in CKD patients with diabetes mellitus.

[0084] Based on this, the protein of interest was screened and verified by molecular biology.

Embodiment 3

[0085] Example 3 SAA1 is a potential marker of Henoch-Schonlein Purpura (HSP) nephritis

[0086] 1. Main experimental materials:

[0087] Hematuria (HSP group, AKI type, 50 cases) and kidney biopsy samples (7 cases) in HSP renal injury patients with diabetes mellitus infection; hematuria in HSP renal injury patients without diabetes (HSP-non-diabetic group, AKI type, 30 cases) ;Co-infected with diabetes; Chronic kidney injury (CKD) crowd urine (50 cases) and kidney puncture samples (8 cases); Thrombocytopenic purpura (ITP) renal injury patients urine (AKI type, 50 cases) and kidney puncture samples Samples (7 cases); urine from healthy people (50 cases). All specimens were collected with the informed consent of the patients and signed informed consent forms.

[0088] 2. Urine specimen collection:

[0089]The hematuria samples of the Henoch-Schonlein Purpura Nephritis group, the Chronic Kidney Injury and Thrombocytopenic Purpura group were collected in the morning of the sec...

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Abstract

The invention relates to a novel allergic purpura nephritis disease marker SAA1, and researches on its application. The present invention proves that the changes of individual serum and urine SAA1 have high sensitivity and specificity for the diagnosis of Henoch-Schonlein Purpura Nephritis in clinical experiments, and it is found that the marker is effective in Henoch-Schonlein Purpura, Thrombocytopenic Purpura, and Chronic Kidney Injury have obvious differences. The invention will provide a new diagnostic method for clinical diagnosis of Henoch-Schonlein Purpura (nephrosis), and has certain potential clinical value in assisting the treatment of Henoch-Schonlein Purpura (Kidney disease) and monitoring the disease course.

Description

technical field [0001] The present invention relates to the field of medical diagnosis, in particular to the application of a SAA1 detection agent in the preparation of a kit for diagnosing Henoch-Schonlein Purpura Nephritis. Background technique [0002] Henoch-Schonlein purpura (HSP) is an inflammatory disease involving small blood vessels throughout the body. Clinically, it mainly manifests as involvement of the skin, gastrointestinal tract, joints, and kidneys, and may also involve the brain, lungs, and scrotum. The pathological feature is that there are immunoglobulin A (immunoglobulin A, IgA)-based immune complex deposition (immunofluorescence) on the small blood vessel walls of the affected organs, and allergic purpura nephritis (Henoch-Schonlein purpuranephritis, HSPN) It refers to renal parenchymal damage caused by Henoch-Schonlein Purpura. Hematuria and / or proteinuria can be diagnosed clinically as Henoch-Schonlein Purpura during the course of Henoch-Schonlein Purp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2800/347
Inventor 何强金娟李一文龚建光赵黎
Owner ZHEJIANG PROVINCIAL PEOPLES HOSPITAL
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