Application of bleomycin in the preparation of drugs for inhibiting tooth eruption

A technology of bleomycin and drugs, which is applied in the field of pharmaceutical invention patents, can solve the problems of large peripheral soft and hard tissue trauma, subjective pain, patient fear, etc., and achieve the reduction of physical and mental pain, good clinical application potential and value, and good biological safety effect

Active Publication Date: 2021-04-13
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the complexity of the operation process, the large trauma to the surrounding soft and hard tissues, the severe postoperative reaction, the patient's fear, and the subjective feeling of pain are difficult to overcome with current surgical treatment.

Method used

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  • Application of bleomycin in the preparation of drugs for inhibiting tooth eruption
  • Application of bleomycin in the preparation of drugs for inhibiting tooth eruption
  • Application of bleomycin in the preparation of drugs for inhibiting tooth eruption

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Effects of Bleomycin (BLM) on human dental follicle stem cells:

[0034] The steps are as follows:

[0035] (1) Dilute the prepared bleomycin preparation to the corresponding drug concentration of 0.1mU / ml, 1mU / ml, 10mU / ml with dental follicle stem cell medium, and first use normal medium to culture human dental follicle stem cells (hDFSCs) , after human dental follicle stem cells adhered to the wall, the above-mentioned diluted bleomycin-containing medium was replaced, and the hDFSCs cell viability, cell cycle and expression of cell surface markers were detected after bleomycin treatment for 12 hours and 24 hours.

[0036] The results suggested that there was no significant statistical difference in the cell viability, cell cycle and cell surface markers of hDFSCs after treatment with different concentrations of BLM ( figure 1 Middle A-C).

[0037] (2) The stem cell differentiation ability of hDFSCs after treatment with different concentrations of BLM was detected. ...

Embodiment 2

[0043] Bleomycin (BLM) inhibits tooth eruption in Wistar rats:

[0044] The steps are as follows:

[0045] (1) Bleomycin preparations at concentrations of 0.2 U / kg, 0.5 U / kg, and 2 U / kg were injected into the dental follicle of the left mandibular first molar of 7-day-old Wistar rats, and the same volume dose was injected into the corresponding position on the right side Phosphate buffer saline (PBS) was used as a control, and the same drug dose and concentration were injected twice at the same location with an interval of one day.

[0046] (2) The rats in each group were euthanized 1 week, 2 weeks and 3 weeks after the completion of drug injection, and the mandibular specimens were taken for photographic record and X-ray examination. Complete eruption in 2-3 weeks, and the mandibular first molars after injection of 0.5U / kg and 2U / kg concentration of bleomycin preparations were still embedded in the original position in the jaw until the end of the observation ( Figure 4 In...

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Abstract

The present invention relates to the field of pharmaceutical invention patents, and discloses the application of bleomycin in the preparation of drugs for inhibiting tooth eruption. The applicant found that local application of appropriate concentration of bleomycin in the targeted dental follicle can inhibit the tooth eruption The role of it, so that it continues to be located in the jaw, to prevent the adjacent soft and hard tissue diseases it induces. The application of bleomycin to inhibit tooth eruption has a definite effect and has good biological safety. It provides a minimally invasive, comfortable and easy-to-operate new method for the clinical prevention and treatment of impacted teeth or impacted teeth and their complications .

Description

technical field [0001] The present invention relates to the field of pharmaceutical invention patents, and more specifically relates to the application of bleomycin in the preparation of drugs for inhibiting tooth eruption. The technical solution of the present invention provides a micro- Innovative, comfortable and easy-to-use new method. Background technique [0002] Impacted teeth or impacted teeth and their induced oral and maxillofacial inflammation and tumors are the most common diseases in oral and maxillofacial surgery, and they often occur in the upper and lower third molars, often leading to irreversible damage to adjacent soft and hard tissues or infection in adjacent spaces , and may induce jaw tumors. Potential interfering factors such as impacted teeth or impacted teeth need to be removed preventively due to orthodontic or dental implant restoration treatment plans. [0003] At present, clinical treatment methods for impacted teeth or impacted teeth mainly in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/14A61P1/02A61P29/00A61P25/00A61P35/00
CPCA61K38/14A61P1/02A61P25/00A61P29/00A61P35/00
Inventor 任建岗王海涛赵吉宏王竟楠李志政夏厚福蔡育
Owner WUHAN UNIV
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