Ocean source sporidiobolus pararoseus and application thereof in preventing and controlling strawberry diseases
A lock throwing and yeast technology, which is applied in the application field of marine source lock throwing yeast and its prevention and control of strawberry diseases, can solve the problems of mechanical damage, perishability, etc., achieve strong tolerance, reduce rot loss, and inhibit infection Effect
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specific Embodiment 1
[0021] 1. Separation and purification of strains
[0022] Take sea mud from the mangrove forest in Zhangzhou, Fujian, weigh 1g sea mud sample in 100mL sterile water, mix it on a vortex instrument, and then carry out gradient dilution to 10 -2 、10 -3 and 10 -4 , and then pipette 1 mL of the diluted solution and spread it on the nutritional yeast dextrose agar (Nutrient yeast dextrose agar, NYDA) medium, and culture it upside down at 28°C for 2 days. Pick colonies that are visible to the naked eye, transfer them to NYDA plates, and cultivate them at constant temperature until a single colony is purified, then transfer the colonies to NYDA medium for storage.
[0023] Preparation method of NYDA selective medium: weigh 20 g of agar, 8 g of beef extract, 5 g of yeast extract and 10 g of glucose, dissolve in 1 L of distilled water, sterilize under high temperature and high pressure at 121 °C for 15 min, and wait for the medium to cool to 55 °C Add 1% streptomycin to the medium ma...
specific Embodiment 2
[0027] The antagonism of S. cinereus against Botrytis cinerea in vitro and in vivo
[0028] The antagonism of marine-derived S. cinerea ZMY-1 against Botrytis cinerea was tested on potato dextrose agar (PotatoDextrose Agar, PDA) medium and strawberry fruit.
[0029] In vitro antagonism test: Botrytis cinerea was cultured on a PDA plate for 7 days in advance, and a 5 mm diameter bacterial block was punched out with a sterilized hole punch. In the control group, the Botrytis cinerea block was directly placed in the center of the newly prepared PDA plate, and in the yeast treatment group, 100 μL of 1×10 8 Cells / mL suspension of Septoria sp. ZMY-1 was spread evenly, and then the Botrytis cinerea block was placed in the center, and cultured in a constant temperature and humidity incubator at 25°C for 3 days, and the plaque diameter was measured.
[0030] In vivo antagonism test: pre-culture Botrytis cinerea on PDA plate for 7 days, wash the spores with sterile water, and prepare ...
specific Embodiment 3
[0032] Spray before harvest
[0033] Inoculate the above-mentioned Septoria sp. ZMY-1 on NYDA medium, culture it at 28°C for 2 days, inoculate it on the plate with an inoculation loop on 200mL liquid NYDB medium, cultivate it at 28°C 200rpm for 48 hours, and dilute it with sterile water. Prepared to a concentration of 1 x 10 8 cells / mL bacterial suspension.
[0034]Spray at the early red stage, half-red stage and red ripe stage of strawberries, that is, 6 days, 3 days and 1 day before harvesting. The treatment group is sprayed with the suspension of Septoria sp. ZMY-1, and the control group is sprayed with distilled water. Pick the fruit when it is ripe, transport the fruit back to the laboratory, store it at shelf temperature and low temperature, and observe the occurrence of diseases.
[0035] Such as Figure 4 and Figure 5 As shown, the incidence of disease of strawberries treated with preharvest spraying Septoria sp. ZMY-1 was only 25.5% when stored at shelf temperat...
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