Method of capturing genomic target sequence for high-throughput sequencing

A target sequence and genome technology, applied in the field of high-throughput sequencing to capture genome target sequences, can solve problems such as short validity period, compressed gene sequencing service profit margin, and unsuitability for single-gene genetic diseases
CN109097443AActive Publication Date: 2018-12-28SUZHOU GENESCI CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SUZHOU GENESCI CO LTD
Publication Date
2018-12-28

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Abstract

The invention discloses a method of capturing a genomic target sequence for high-throughput sequencing. The method can sequentially include the following steps of (1) preparing a genomic DNA library;(2) hybridizing with a capture probe library, wherein the capture probe library is composed of n specific probes, n is a natural number, and the specific probes are single-stranded DNA molecules whichare designed and synthesized according to the nucleotide sequence of the target sequence and subjected to locked nucleic acid modification. The capture probe library is composed of 440 probes including the LNA probe-1 to the LNA probe-440; the nucleotide sequences of the LNA probe-1 to the LNA probe-440 are sequentially shown as sequence 1 to sequence 440 in a sequence list. Experiments prove that the capture probe library can capture the corresponding target sequences in a BRCA1 gene and a BRCA2 gene. Therefore, the provided method has important application value.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a method for capturing genome target sequences for high-throughput sequencing. Background technique

[0002] Since the completion of the human genome map, governments of various countries have increased their support for human genome research. Under such a background, gene sequencing technology has developed rapidly, and the gene sequencing market has also expanded rapidly, and has already moved away from the laboratory. into the clinic. Predict your future health status through genetic testing, carry out targeted health care and treatment, and move from passive preventive treatment to proactive health prediction. In the developed countries of the United Kingdom and the United States, genetic testing is as popular as physical examinations. There are more than 6,000 genetic testing items registered by the National Health Service of the United States. The number of genetic testing peo...

Claims

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