CircRNA marker used for breast cancer diagnosis and application of marker

A breast cancer and forward primer technology, applied in the field of biomedicine, can solve problems such as circRNA in whole blood of breast cancer that have not yet been seen

Active Publication Date: 2018-12-28
SHANDONG UNIV QILU HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, the inventor has not seen the report of circRNA in whole blood of breast cancer as a marker

Method used

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  • CircRNA marker used for breast cancer diagnosis and application of marker
  • CircRNA marker used for breast cancer diagnosis and application of marker
  • CircRNA marker used for breast cancer diagnosis and application of marker

Examples

Experimental program
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Effect test

Embodiment 1

[0048] A detection kit for diagnosing breast cancer, the detection kit, detection system includes a reverse transcription reaction system and qPCR reaction system, the detection kit includes reagents for preparing the reverse transcription reaction system and for Prepare reagents for qPCR reaction system.

[0049] The reagents used to prepare the reverse transcription reaction system include at least reverse transcription buffer, dNTP mixed solution, RNase protein inhibitor, reverse transcriptase solution and the like.

[0050] The reagents used to prepare the qPCR reaction system include at least forward and reverse primers for one or more combinations of circ_0000745, circ_0001531, and circ_0001640, as well as forward and reverse primers for internal reference genes, SYBR Green mixed solution, nuclease-free pure water, etc. See Table 1 for the relevant primer sequences.

Embodiment 2

[0052] (1) Clinical samples

[0053] From June 27, 2016 to August 30, 2017, peripheral blood from 68 breast cancer patients and 13 healthy controls were recruited at Qilu Hospital of Shandong University. All patients and controls were Han females.

[0054] Fasting venous whole blood (2ml) was collected and untreated whole blood samples were immediately stored in liquid nitrogen until further analysis. All breast cancer blood samples were collected before surgery or any treatment. Control samples were collected from healthy females with no past or present history of malignancy or inflammation. All participants obtained written informed consent. This study was approved by the Ethics Committee of Qilu Hospital of Shandong University.

[0055] (2) RNA extraction

[0056] Take 250ul of whole blood and transfer it to a 1.5ml centrifuge tube, add 750μl of TRIzol LS Reagent and 20μl of glacial acetic acid, shake the tube vigorously by hand until mixed. After homogenization, the ...

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Abstract

The invention relates to a circRNA marker used for breast cancer diagnosis and an application of the circRNA marker. Compared with healthy control groups, it is found that expression of 3 kinds of circRNA (hsa_circ_0000745, hsa_circ_0001531 and hsa_circ_0001640) in whole blood of breast cancer is up-regulated. ROC curve analysis displays that the marker can better distinguish breast cancer patients and healthy people, and the 3 kinds of circRNA have the AUCs (area under curve) of 0.7998, 0.8253 and 0.7161 respectively, the sensitivity of 48.53%, 92.65% and 73.53% respectively and the specificity of 100%, 69.23% and 69.23% respectively.

Description

technical field [0001] The disclosure belongs to the field of biomedicine, and in particular relates to a circRNA marker for breast cancer diagnosis and its application. Background technique [0002] The statements herein merely provide background information related to the present disclosure and may not necessarily constitute prior art. [0003] Breast cancer is a malignant tumor that occurs in the glandular epithelial tissue of the mammary gland, which seriously threatens women's health. The occurrence and development of breast cancer are related to the abnormal expression of many tumor markers. At present, there are many breast cancer tumor markers: progesterone receptor (PR), vascular endothelial growth factor (VEGF), estrogen receptor (ER ), CD44, p53, etc. But its real clinical application is very limited. Therefore, it is very important and urgent to further develop new non-invasive biomarkers for detecting breast cancer with higher sensitivity and specificity. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/158C12Q2600/178
Inventor 张凯马榕王亚文朱江陈旭
Owner SHANDONG UNIV QILU HOSPITAL
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