Artificial auxiliary mating and breeding method for dendroctonus armandi
An artificially assisted Huashan pine technology, applied in the field of plant protection, can solve the problems of high requirements, time barriers, shortening the research cycle of preventing and controlling Huashan pine beetles, etc., and achieve the effect of shortening the scientific research cycle
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[0013] Below in conjunction with embodiment the present invention is described further as follows:
[0014] a. Take a healthy section of Huashan pine, diameter 25cm, length 1.2m, drill a hole on the side to the xylem, the hole diameter is 0.8cm, insert 10cm into the sandy soil of the flowerpot;
[0015] B. put the Huashan pine beetle female into the transparent capsule of cassava starch material with a diameter of 0.8cm and a length of 2cm, insert the open end of the transparent capsule into the hole drilled on the Huashan pine section, and then place the flower pot in the darkroom; Males and females of Huashan pine beetle can be distinguished by wing rubbing sound, those who can make wing rubbing sound are males, and those without rubbing sound are females, males can only be identified once, and females can be identified three times; Anatomical verification of 50 worms and 50 male worms each. The correct rate of male worms is 100% once, the correct rate of female worms is 80%...
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