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Artificial auxiliary mating and breeding method for dendroctonus armandi

An artificially assisted Huashan pine technology, applied in the field of plant protection, can solve the problems of high requirements, time barriers, shortening the research cycle of preventing and controlling Huashan pine beetles, etc., and achieve the effect of shortening the scientific research cycle

Active Publication Date: 2019-02-01
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to prevent and control the Huashan pine beetle more effectively, it is necessary to conduct in-depth research on the survival, breeding and harm of the Huashan pine beetle. However, because the short adult period of the Huashan pine beetle is a time barrier for the research, artificial assisted mating is required. Control research continues to provide insect sources, and shortens the research period for the prevention and control of Huashan pine beetle through subculture breeding of Huashan pine beetle. It can only raise the Huashan pine beetle from larvae to adults, and it is still impossible to use culture medium and artificial feed for subculture in the room. There are no relevant literature reports on the subculture method of Huashan pine beetle. If it can be effectively The subculture of the Huashan pine beetle indoors will buy more time for the research of the Huashan pine beetle, which is conducive to the effective prevention and control of the Huashan pine beetle

Method used

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Effect test

Embodiment Construction

[0013] Below in conjunction with embodiment the present invention is described further as follows:

[0014] a. Take a healthy section of Huashan pine, diameter 25cm, length 1.2m, drill a hole on the side to the xylem, the hole diameter is 0.8cm, insert 10cm into the sandy soil of the flowerpot;

[0015] B. put the Huashan pine beetle female into the transparent capsule of cassava starch material with a diameter of 0.8cm and a length of 2cm, insert the open end of the transparent capsule into the hole drilled on the Huashan pine section, and then place the flower pot in the darkroom; Males and females of Huashan pine beetle can be distinguished by wing rubbing sound, those who can make wing rubbing sound are males, and those without rubbing sound are females, males can only be identified once, and females can be identified three times; Anatomical verification of 50 worms and 50 male worms each. The correct rate of male worms is 100% once, the correct rate of female worms is 80%...

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Abstract

The invention provides an artificial auxiliary mating and breeding method for dendroctonus armandi, which comprises the following steps of: drilling a hole to the xylem on the side surface of a section of armandiand inserting the section of armandi into the sand soil of a flowerpot; placing the female dendroctonus armandi into a transparent capsule, embedding the capsule into the hole drilled in the pine section, and placing the flowerpot in a dark room; taking out the transparent capsule upon observing and determining that the female dendroctonus armandi intrudes into the pine section of thePinus armandii, and embedding the transparent capsule of the male dendroctonus armandi into the hole; After all male dendroctonus armandi are observed and determined to invadethe the section of armandi, the flowerpot is moved out of the dark room to be cultured, and the bark is watered and kept moist until the dendroctonus armandi of second generation emerge and fly. The artificial auxiliary mating and breeding method for dendroctonus armandi is capable of solving the problem of difficult indoor generation breeding of dendroctonus armandi and continuously providing a insect source for prevention and control research of dendroctonus armandi, thereby shortening the scientific research period. According to artificial auxiliary mating and breeding method for dendroctonus armandi, 30 days afterthe male and female dendroctonus armandi are judged to be successfully mated by artificial passage, the second generation of dendroctonus armandi in a larvae gallery is mostly in the larval stage ofdifferent ages. After 75 days of successful mating, adults are gradually raised and flying, and the success rate of male and female mating is 75%.

Description

technical field [0001] The invention relates to an artificially assisted mating and raising method, in particular to a method for artificially assisting mating and raising of the Huashan pine beetle, and belongs to the field of plant protection. Background technique [0002] Bark beetles are a serious pest in forest areas. At present, more than 6,000 species are known in the world, and there are more than 500 species in China. 70% of bark beetles harm the phloem and xylem of coniferous trees. The stage is hidden under the bark, and there is only a short-term flying and spreading in the adult stage. Due to the unique life history and hazard characteristics of bark beetles, it greatly increases the difficulty of management. [0003] The Huashan pine beetle belongs to the Coleoptera (Coleoptera) Scolytidae (Scolytidae) genus ( Dendroctonus ), is a kind of bark beetle that is more harmful. It is mainly distributed in Shaanxi, Sichuan, Gansu, Henan and other places in my countr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 陈辉赵明振王洪琳郑洁
Owner SOUTH CHINA AGRI UNIV
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