Marker screening method for beef origin place and application thereof

A screening method and marker technology, applied in the field of marker screening in beef origin, can solve the problems of lack of research on identification methods

Active Publication Date: 2019-02-01
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still a lack of research on the identification

Method used

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  • Marker screening method for beef origin place and application thereof
  • Marker screening method for beef origin place and application thereof
  • Marker screening method for beef origin place and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0084] 1. Sample information

[0085] The samples were freeze-dried beef powder, including: 30 samples from Australia, 30 samples from China, 10 samples from Canada, 11 samples from New Zealand, and 8 samples from Uruguay.

[0086] 2. Sample pretreatment

[0087] Take 2g of each portion of beef powder, use Folch solvent (chloroform / methanol 2:1, 1mL), ultrasonicate for 3min, centrifuge at 10 000r / min for 30min, add 1mL of water to the supernatant, centrifuge at 5000r / min for 15min, and remove the lower layer of lipid. Nitrogen was blown, and then the sample to be tested was obtained after redissolving to 1 mL with Folch solvent.

[0088] 3. UPLC-HRMS

[0089] Liquid phase conditions: Agilent 1290UPLC, Agilent EC C18 column (3.0×150, 2.7μm);

[0090] Mobile phase A, acetonitrile: water (6:4), 5mM ammonium acetate; mobile phase B, isopropanol: acetonitrile (9:1), 5mM ammonium acetate;

[0091] Conditions: 0~3min, 35% phase B, 3~8min, phase B rises from 35% to 60%, 8~16min, p...

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Abstract

The invention relates to the field of beef origin place identification, in particular to a marker screening method for beef origin place and application thereof. The method comprises the following steps of: 1) using a chromatography-mass spectrometer to perform detection on lipid samples of the beef from different origin places; 2) analyzing the detection results by adopting a non-targeted lipid profile analysis and a principal component analysis; 3) performing significant difference analysis on the analysis results to obtain difference factors among lipids of the beef of different origin places; and 4) comparing the difference factors with a lipidomics database to obtain a marker. The screening method is based on the analysis of non-targeted lipidomics, and adopts the stoichiometry to analyze a difference marker. In this way, the markers selected by the method are used for identification of the origin place, and the method has high accuracy.

Description

technical field [0001] The invention relates to the field of beef origin identification, in particular to a marker screening method for beef origin and its application. Background technique [0002] In recent years, with the rapid development of the global economy, people's living standards have improved. At the same time, due to frequent food safety issues, people's focus of life has shifted from food and clothing to nutrition, health and safety. As a commodity, food flows in the market, and the competition for interests prompts some merchants to take risks regardless of food quality, which leads to many food safety problems. The main function of food safety traceability is to trace back to the link where the problem occurred in time when a food safety problem occurs; it can promptly recall the food with the problem and monitor and identify some behaviors. [0003] Beef is one of the most commonly used meats. The rapid growth of per capita beef consumption and the rapid de...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 徐贞贞徐雷王雪陈爱亮杨曙明
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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