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Culture dish device and using method thereof

A culture dish and dish cover technology, which is applied in the field of cell culture, achieves the effects of low cost, simple structure and reduction of cell contamination problems

Inactive Publication Date: 2019-02-22
国家康复辅具研究中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The first technical problem to be solved by the present invention is to provide a petri dish device, which can directly apply static or dynamic mechanical stimulation to the cells in the petri dish, so as to facilitate and better study the influence of compressive stress on the cells, and facilitate With the help of mechanical devices, the cells in the culture dish can be pressurized to reduce the problem of cell contamination during operation, with simple structure and low cost

Method used

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  • Culture dish device and using method thereof
  • Culture dish device and using method thereof
  • Culture dish device and using method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0128] Before the cells in the dish are normally cultured and pressurized, the dish cover 2 is only gently buckled, and a common marketed dish cover 2 can also be used. Replace the dish cover 2 provided with the air pressure regulating part 3 designed in the present invention under aseptic conditions, and make the plunger 36 reach the scale line 10 by twisting the knob 38, so as to pressurize the cells in the culture dish by 10Kpa, It is convenient to study the mechanical effect of cells under the static pressure stimulation of 10Kpa.

Embodiment 2

[0130] Before the cells in the dish are normally cultured and pressurized, the dish cover 2 is only gently buckled, and a common marketed dish cover 2 can also be used. Replace the dish cover 2 provided with the air pressure regulating part 3 designed in the present invention under sterile conditions, and manually push the push-pull handle 33 at a frequency of 1 Hz to make the plunger 36 reciprocate, so as to continuously pressurize the cells in the culture dish , which is convenient for studying the mechanical effect of cells under the stimulation of dynamic pressure.

Embodiment 3

[0132] Before the cells in the dish are normally cultured and pressurized, the dish cover 2 is only gently buckled, and a common marketed dish cover 2 can also be used. Under aseptic conditions, the dish box 1 is placed on the box body 41 of the air pressure regulating drive device 4 of the present invention, the dish cover 2 is covered, and the frequency of the dynamic pressure is precisely controlled by the control panel 11 to be 1Hz and the size is 10Kpa, so as to realize the adjustment of the petri dish. The cells inside are continuously pressurized, which is convenient for studying the mechanical effects of cells under dynamic pressure stimulation.

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Abstract

The invention discloses a culture dish device and a using method thereof. The culture dish device comprises a dish box and a dish cover which are in seal connection, and an air pressure regulating assembly is arranged on the dish cover. The device can directly apply static or dynamic mechanical stimulation to cells in the culture dish, the influence of compressive stress on the cells can be studied better and conveniently, the cells in the culture dish can be conveniently compressed by a mechanical device, cell pollution brought by the operation process is reduced, and the device has simple structure and low cost. The using method comprises the step of culturing the cells under pressure higher than 101 KPa, or culturing the cells under pressure lower than 101 KPa, or culturing the cells indynamic pressure. Great convenience is provided for studying biomechanical effects of the cells under different kinds of pressure.

Description

technical field [0001] The invention belongs to the field of cell culture, and in particular relates to a culture dish device and an application method thereof. Background technique [0002] Petri dish is a kind of biological consumables used for cell culture, which can be used in the laboratory to study cell biological behavior. Existing petri dish is made up of dish box (1) and dish cover (2), and described dish box (1) is surrounded by bottom plate and side plate to form cylindrical space, and described dish cover (2) is surrounded by top plate and side plate. Form a cylindrical space, the dish box (1) is compatible with the dish cover (2), and the dish cover (2) is fastened to the dish box (1). This petri dish has a single structure and does not It's also functional. Cells in the body are in a complex mechanical environment, and mechanical stimulation plays an important role in regulating the shape, growth, proliferation and function of cells. In the experiment of cel...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12M1/22C12M1/00
CPCC12M23/10C12M35/04
Inventor 张莹莹宋庆贺李剑邢阳辉樊瑜波
Owner 国家康复辅具研究中心
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