Artificial cellulosome, method for decomposing cellulose by using cellulosome and producing alcohol

A cellulose and cellulolytic enzyme technology, applied in biochemical equipment and methods, fixed on or in biological cells, enzymes, etc., can solve problems such as short replication cycle, and achieve the effect of high cellulolytic activity

Active Publication Date: 2019-04-30
朱一民 +2
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the above deficiencies, the present invention provides a cellulosome using recombinant phage as a protein scaffold, which can link hundreds to thousands of cellulolytic enzymes on the surface of a single phage by self-assembly and anchor to the surface of Saccharomyces cerevisiae, not only overcoming Due to the current problem of decomposition capacity, artificial cellulosomes that can be produced at high speed can be obtained through the extremely short replication cycle of bacteriophage itself, providing a high-efficiency cellulolysis platform

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Artificial cellulosome, method for decomposing cellulose by using cellulosome and producing alcohol
  • Artificial cellulosome, method for decomposing cellulose by using cellulosome and producing alcohol
  • Artificial cellulosome, method for decomposing cellulose by using cellulosome and producing alcohol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0030] In the prior art, artificially manufactured cellulosomes are usually directly bonded with decomposing enzymes to protein scaffolds to imitate the structure of natural cellulosomes. However, the number of enzymes that can be bonded cannot reach the equivalent number of natural cellulosomes , causing the decomposition ability to be less than expected. In the present invention, peptides with smaller molecular weights are bonded to protein scaffolds, and then the peptides are connected to decomposing enzymes, which overcomes steric obstacles and other unfavorable factors, so that a large number of decomposing enzymes can be bonded to a single protein scaffold. In addition, the inventors chose phage as the backbone of the protein scaffold of the artificial cellulosome, and expressed a large amount of peptides on the surface of the phage through genetic recombination technology, thereby increasing the engagement of decomposing enzymes.

[0031] In the present invention, recom...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a cellulose decomposition method, which is characterized in that a recombinant bacteriophage is created, by modifying the genome of the bacteriophage, the surface of the bacteriophage is expressed as follows: a conjugated peptide having affinity with a clastic enzyme, and an anchor peptide having affinity with anchor positioning on a surface of the cell; the clastic enzyme is contacted with the recombinant bacteriophage; and the cellulose is contacted with the clastic enzyme.

Description

technical field [0001] The present invention relates to a method for decomposing cellulose, in particular to a method for decomposing cellulose by cellulosomes with phage scaffolds and a method for producing alcohol by using the method. Background technique [0002] Among the known bio-alcohol production processes, consolidated bioprocessing (CBP) is currently the best process. By integrating all the reactions in the production process into the same strain (for example: Saccharomyces cerevisiae), the production can be greatly reduced. costs and needs. Among them, the cellulosomes found in the anaerobic Clostridium thermocellum (C.thermocellum) are regarded as one of the key technologies in the development of CBP procedures. [0003] The cellulosome is mainly composed of a protein scaffold structure, and a variety of cellulolytic enzymes are combined with the scaffold structure to form a complex with multiple decomposition functions. The dilemma of limited expression on the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N11/16C12P19/14C12P19/02C12P7/10
CPCC12N9/2437C12N11/16C12P7/10C12P19/02C12P19/14C12Y302/01004C12Y302/01091Y02E50/10
Inventor朱一民魏毓宏蔡伸隆
Owner朱一民