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New species Xylariaceae sp. DPZ-SY43 of mangrove rhizosphere cellulose degrading fungi and application thereof

A DPZ-SY43, degrading bacteria technology, applied in the production of cellulase and the preparation of bio-organic matter-degrading bacterial fertilizer, the field of new species of mangrove rhizosphere cellulose-degrading fungi, can solve the problem of large environmental pollution and energy efficiency lower problem

Inactive Publication Date: 2012-03-14
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

my country is a large agricultural country, which can produce a large amount of straw cellulose every year, but it is mainly used through the traditional simple incineration method, the energy utilization rate is extremely low, and the environmental pollution is relatively large

Method used

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  • New species Xylariaceae sp. DPZ-SY43 of mangrove rhizosphere cellulose degrading fungi and application thereof
  • New species Xylariaceae sp. DPZ-SY43 of mangrove rhizosphere cellulose degrading fungi and application thereof
  • New species Xylariaceae sp. DPZ-SY43 of mangrove rhizosphere cellulose degrading fungi and application thereof

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Experimental program
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Effect test

Embodiment 1

[0021] 1. Materials and methods

[0022] 1. Materials

[0023] 11 Soil sample collection

[0024] The samples were collected from the rhizosphere sediments of Rhizophora stylosa in the mangrove area along the Hongsha River in Sanya City, Hainan Province. After the samples were collected, they were put into sterilized sealed polyethylene bags and brought back to the laboratory for storage at 4°C.

[0025] 1.2 Medium

[0026] 1.2.1 Initial screening medium (g / L):

[0027] The preparation method of each liter of primary screening medium is as follows: Potato juice (peel the potatoes, dig out the bud eyes, wash, and slice. Weigh 200g and put it into 1000ml tap water, boil with a slow fire for 30min, filter with double gauze, add water to the filtrate to 1000ml) 800ml, sucrose, 20.0g; NaCl, 1.5g; agar, 15g, soil extract, 200ml; after sterilizing at 121°C, cool the medium to 60°C and add filter-sterilized 100μg / ml ampicillin and 100μg / ml 1ml each of streptomycin sulfate.

[002...

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Abstract

The invention discloses a new species Xylariaceae sp. DPZ-SY43 of mangrove rhizosphere cellulose decomposed fungi and application thereof. Xylariaceae sp. DPZ-SY43 is preserved in the China General Microbiological Culture Collection Center (CGMCC) with a preservation number of CGMCC No:4871 on May 16, 2011 at Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 West Beichen Road, Chaoyang District, Beijing, China. The fungus has the activity of producing cellulase, can be used for producing cellulase, and thus has important value in production and utilization of cellulase. Furthermore, the Xylariaceae sp. DPZ-SY43 can be used for preparing biological organic matter degrading bacterial manure.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a new species of mangrove plant rhizosphere cellulose-degrading fungus Xylariaceae sp.DPZ-SY43 and its application in producing cellulase and preparing bio-organic matter-degrading bacterial fertilizer. Background technique [0002] Mangrove ecosystems are complex and unique ecosystems located in the intertidal zone of tropical and subtropical coasts, including rich animal communities, mangrove plant communities, and microbial communities, which can be maintained in coastal natural marine ecosystems. Higher productivity level is one of the important wetland ecosystem types in the coastal zone (Holguin et al., 2001; Cao Qimin et al., 2008). Because it is located at the junction of ocean and land, its intertidal habitat is highly salinized, the soil is anoxic, high light radiation and periodic seawater inundation, so it contains a large number of unique microorganis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N9/42C05F11/08C12R1/645
Inventor 董俊德潘虎张燕英凌娟陈蕾张偲龙丽娟
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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