New species of Rhizophora stylosa root cellulose degrading fungus Hypoxylon sp. DPZ-SYz-36 and application thereof

A dpz-syz-36, degrading bacteria technology, applied in the biological field, can solve the problem of less cellulase

Inactive Publication Date: 2012-02-01
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, there are some protozoans and bacteria that can also decompose cellulosic substances, but because the amount of cellulase secreted by bacteria is small, and the enzymes produced are intracellular enzymes or adsorbed on the cell wall, so bacteria are rarely used in industry. Cellulase producing strains

Method used

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  • New species of Rhizophora stylosa root cellulose degrading fungus Hypoxylon sp. DPZ-SYz-36 and application thereof
  • New species of Rhizophora stylosa root cellulose degrading fungus Hypoxylon sp. DPZ-SYz-36 and application thereof
  • New species of Rhizophora stylosa root cellulose degrading fungus Hypoxylon sp. DPZ-SYz-36 and application thereof

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Embodiment 1

[0022] 1. Materials

[0023] 1.1 Soil sample collection

[0024]The samples were collected from the rhizosphere sediments of Rhizophora stylosa in the mangrove area along the Hongsha River in Sanya City, Hainan Province in September 2010. After the samples were collected, they were put into sterilized sealed polyethylene bags and brought back to the laboratory at a low temperature of 4°C. save.

[0025] 1.2 Medium

[0026] 1.2.1 Initial screening medium:

[0027] The preparation method of each liter of primary screening medium is as follows: Potato juice (peel the potatoes, dig out the bud eyes, wash, and slice. Weigh 200g and put it into 1000ml tap water, boil with a slow fire for 30min, filter with double gauze, add water to the filtrate to 1000ml) 800ml, sucrose, 20.0g; NaCl, 1.5g; agar, 15g, soil extract, 200ml; after sterilizing at 121°C, cool the medium to 60°C and add filter-sterilized 100μg / ml ampicillin and 100μg / ml 1ml each of streptomycin sulfate.

[0028] 1.2....

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Abstract

The invention discloses a new species of a Rhizophora stylosa root cellulose degrading fungus Hypoxylon sp.DPZ-SYz-36 and application thereof. The Hypoxylon sp. DPZ-SYz-36 was collected in China General Microbiological Culture Collection Center (CGMCC) on May 16, 2011 with the collection number of CGMCC No.4872. The Hypoxylon sp. DPZ-SYz-36 has cellulase producing activity, can be used for producing cellulase, has important value for production and utilization of the cellulase, and can be further used for preparing biological organic matter degrading bacterial fertilizers.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a new hypoxylon sp.DPZ-SYz-36, a rhizosphere cellulose-degrading fungus species of Lavender and its application in the production of cellulase and the preparation of bio-organic matter-degrading bacterial fertilizer. Background technique: [0002] Mangroves are woody plant communities naturally distributed in the intertidal zone of tropical and subtropical coasts, covering about 60-75% of tropical and subtropical coasts, playing an important role in maintaining the ecological balance of bays and estuaries, and are an important part of marine productivity (Holguin et al., 2001; Longhan et al., 2005), considered to be one of the ecosystems with higher productivity. The ecosystem has high levels of organic matter (100 Tg C per year of global litter), providing organic matter to the adjacent coastal waters and associated biome habitats (Holguin et al., 2001; Lugomela and Berg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N9/42C05F11/08C12R1/645
Inventor 董俊德潘虎张燕英凌娟陈蕾张偲龙丽娟
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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