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Method for preparing yeast by adding traditional Chinese medicines in mould fusant

A technology of traditional Chinese medicine and mold, which is applied in mold fusion, traditional Chinese medicine koji making, and added to the field of traditional Chinese medicine koji making, which can solve the problems of heavy workload and tedious work, and achieve the effect of improving the activity of liquefaction enzyme and improving the conversion rate

Active Publication Date: 2014-06-11
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For the fusion technology of protoplasts, traditional methods mostly use drug resistance markers or auxotrophic markers to mark parental strains in order to detect fusion sub-strains, which is quite cumbersome and has a large workload [Chaetomium interspecies with antibiotic resistance as a selection marker Protoplast Fusion. Journal of East China University of Science and Technology (Natural Science Edition). 2010, 36(1): 36-41.]

Method used

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  • Method for preparing yeast by adding traditional Chinese medicines in mould fusant
  • Method for preparing yeast by adding traditional Chinese medicines in mould fusant
  • Method for preparing yeast by adding traditional Chinese medicines in mould fusant

Examples

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Embodiment 1

[0041] In the present embodiment 1, medium used is PDA eggplant bottle medium; Liquid medium is PDA liquid medium Erlenmeyer flask; Experimental bottle is triangular flask; Chinese medicine used is Chinese wolfberry;

[0042] Embodiment 1 of the present invention is carried out according to the process steps described above, and the specific operation steps are as follows successively:

[0043] (1) Mycelium cultivation and pretreatment:

[0044] The two parents, Aspergillus basilica (ZZH) and Trichoderma viride (TV), were respectively transferred to PDA eggplant bottle medium, and after 30 hours of growth, 10 7 pcs / ml mold spore suspension; inoculate 300 μL mold spore suspension into 50 ml PDA liquid medium in a 250 ml Erlenmeyer flask under sterile conditions, place it in an air-bath shaker at a temperature of 30 ° C and a rotation speed of 100 rpm Cultivate for 18 hours to obtain mycelium culture solution; filter the mycelium culture solution through four layers of sterile ...

Embodiment 2

[0069] The medium used in this embodiment, the liquid medium, and the experimental bottle are the same as in Example 1, and the Chinese medicinal material used is Astragalus membranaceus.

[0070] (1) Mycelium cultivation and pretreatment:

[0071] The two parents, Aspergillus basilica (ZZH) and Trichoderma viride (TV), were respectively transferred to PDA eggplant bottle medium, and after 48 hours of growth, 10 9 Individual / ml mold spore suspension; inoculate 300 μL mold spore suspension into 50 ml PDA liquid medium in a 250 ml Erlenmeyer flask under sterile conditions, and place it in an air bath shaker at a temperature of 32°C and a rotation speed of 120 rpm Shake culture for 24 hours to obtain mycelium culture solution; filter the mycelium culture solution through four layers of sterile mirror paper, rinse with sterile water for 3 times to remove the medium, and then use 0.9mol / L sodium chloride to stabilize The agent was centrifuged and washed 3 times in a refrigerated c...

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Abstract

The invention relates to construction of a mould fusant and a method for preparing yeast by adding traditional Chinese medicines, and belongs to the biotechnical field in light industry. According to the method, protoplasts of parents are fused to obtain the fusant by means of capacities difference of Aspergillus albicans which generates saccharifying enzyme and Trichoderma viride which generates cellulase by way of infusing asymmetric inactivated parents and protoplasts. The infusant is used for preparing yeast by adding traditional Chinese medicines so as to obtain the traditional Chinese medicine yeast with activity of saccharifying enzyme, activity of cellulose and active components of traditional Chinese medicines at the same time. The method comprises the following process steps: cultivation and pre-treatment of mycelium, preparation of protoplast, asymmetric inactivation of protoplast, construction of the fusant, fusant screening, yeast preparation by traditional Chinese medicines and the like. The method provided by the invention is simple to operate and easy to realize, the fusant traditional Chinese medicine yeast obtained is of significance to subsequent utilization of cellulase component in brewing materials and health-care function of the product.

Description

technical field [0001] The invention relates to a traditional Chinese medicine koji-making technology, in particular to a method for adding mold fusogens to traditional Chinese medicine koji-making methods; the method uses asymmetric inactivation technology to construct mold fusogens and adds them to traditional Chinese medicine koji-making Among them, the medicinal koji with glucoamylase activity, cellulase activity and active ingredients of traditional Chinese medicine is obtained, which belongs to the field of light industry biotechnology. Background technique [0002] Medicinal koji is a saccharification starter for brewing and other industries. The production method of traditional medicinal koji is similar to the production method of traditional brewing Daqu or Xiaoqu. The microorganisms in the medicinal koji come from natural inoculation or the breeding of the original koji powder. Through the natural fermentation of the microorganisms, the original characteristics of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/04C12R1/645
Inventor 张文学刘跃红吴正云李丽钟霞罗芳
Owner SICHUAN UNIV
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